27580-13-8 Usage
General Description
NAPHTHOL AS-D PHOSPHATE is a popular and widely used fluorescent stain that is used in various biological and medical applications. It is primarily used in enzyme-linked immunosorbent assays (ELISAs) to detect and quantify alkaline phosphatase activity. The chemical is often used in combination with other substrates to achieve specific detection of the enzyme, making it a valuable tool for research and diagnostic purposes. NAPHTHOL AS-D PHOSPHATE is known for its high sensitivity and stability, making it an essential reagent for the detection of certain enzymes and markers in biological samples.
Check Digit Verification of cas no
The CAS Registry Mumber 27580-13-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,7,5,8 and 0 respectively; the second part has 2 digits, 1 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 27580-13:
(7*2)+(6*7)+(5*5)+(4*8)+(3*0)+(2*1)+(1*3)=118
118 % 10 = 8
So 27580-13-8 is a valid CAS Registry Number.
InChI:InChI=1/C18H16NO5P/c1-12-6-2-5-9-16(12)19-18(20)15-10-13-7-3-4-8-14(13)11-17(15)24-25(21,22)23/h2-11H,1H3,(H,19,20)(H2,21,22,23)
27580-13-8Relevant articles and documents
Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids
Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi
, p. 1347 - 1353 (2007/10/02)
A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.
