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3483-12-3

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3483-12-3 Usage

Chemical Properties

Dithiothreitol (DTT), also known as Cleland's reagent, is a small-molecule redox reagent . Its oxidized form is a disulfide-bonded 6-membered ring. DTT has an epimeric ('sister') compound, dithioerythritol (DTE).DTT is a white crystalline powder. It is highly soluble in water (clear solution, OD<0.05 at 0.02M), but also in ethanol, chloroform, ether and ethyl acetate.DTT is an unusually strong reducing agent, with a redox potential of -0.33 V at pH 7. The pKa of thiol groups is typically ~8.3.The reduction of a typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions.

Uses

Different sources of media describe the Uses of 3483-12-3 differently. You can refer to the following data:
1. Dithiothreitol is a redox reagent commonly used as a reducing agent for thiolated DNA. Dithiothreitol is also used to reduce the disulfide bonds of proteins.
2. DL-dithiothreitol (DTT) is a sulfhydryl compound that acts both as a reagent reducing disulfide bonds and as a protein denaturant on staphylococcal biofilm (Wu et al. 2011). Thanks to these properties, DTT is routinely used in clinical microbiology to liquefy respiratory specimens, but it has also been proven effective to detach biofilm from orthopaedic prosthesis (Drago et al. 2012, 2013). Since it is crucial to discriminate implant-related infections from aseptic loosening in orthopaedics, and because of the difficulty to diagnose subclinical infections, a prompt diagnosis of indwelling device-related infection is important for successful treatments (Borens et al. 2013). A fast microbiological diagnosis and bacterial identification should be recommended in order to set up a specific antimicrobial therapy. According to this strategy, the bacterial detachment from biofilm on explants should be accelerated by supporting the pathogen viability.

Definition

ChEBI: 1,4-dithiothreitol is the threo-diastereomer of 1,4-dimercaptobutane-2,3-diol. It has a role as a reducing agent, a chelator and a human metabolite. It is a dithiol and a 1,4-dimercaptobutane-2,3-diol.

Application

Dithiothreitol (DTT) is a water-soluble reducing reagent used for various applications in biotechnology, biology and biochemistry :reduces quantitatively disulfides, generating sulfhydryls (used typically at 1-10mM for protein SS reduction)reduction of proteins before SDS-PAGE analysis, studies of protein structure and function (Kaji 1993)keep sulfhydryl groups of biomolecules in the reduced state - protects biomolecules in various applications (enzymes or receptors, living cells under ionizing radiations)prevents the fading of fluorescence such as FITC labeled conjugates (Picciolo 1984)

General Description

1,4-Dithiothreitol (DTT) is the threo isomer of 2,3-dihydroxy-1,4-dithiolbutane, and an isomer of 1,4-dithioerythritol. DTT is used in molecular biology to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups, as described by Cleland in his pioneering publication from the 1960's. Its usefulness as an reducing agent stems from its water solubility and reduced odor compared to previous thiol compounds.DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. DTT is less pungent and less toxic than 2-mercaptoethanol. Typically, a 7-fold lower concentration of DTT (100 mM) is required compared to 2-mercaptoethanol (5% v/v, 700 mM).

Biochem/physiol Actions

DL-Dithiothreitol denatures proteins by reducing the disulphide bonds and reducing them to SH groups. It also prevents intramolecular and intermolecular disulfide bond formation between cysteine residues of proteins.

storage

Store lyophilized at 4°C, desiccated. In lyophilized form, the chemical is stable for 12 months. Once in solution, store at -20°C and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.DTT solutions should be prepared fresh daily. If improperly stored (including room temperature and solution forms) its reducing ability may be reduced. Exposure to air should be minimized, even though DTT has a lower tendency to be oxidized directly by air than other reducing agents.Recorded half-life of DTT solutions at various pH and temperatures (all are in M potassium phosphate buffer):pH 6.5 @ 20°C= 40 hourspH 7.5 @ 20°C = 10 hourspH 8.5 @ 20°C = 1.4 hourspH 8.5 @ 0°C = 11 hourspH 8.5 @ 40°C = 0.2 hourspH 8.5 @20°C (+0.1 mM Cu2+) = 0.6 hourspH 8.5 @20°C (+0.1 mM EDTA) = 4 hours

Check Digit Verification of cas no

The CAS Registry Mumber 3483-12-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,4,8 and 3 respectively; the second part has 2 digits, 1 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 3483-12:
(6*3)+(5*4)+(4*8)+(3*3)+(2*1)+(1*2)=83
83 % 10 = 3
So 3483-12-3 is a valid CAS Registry Number.
InChI:InChI=1/C4H10O2S2/c1-2(5)3(6)4(7)8/h2-8H,1H3

3483-12-3 Well-known Company Product Price

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  • TCI America

  • (D1071)  DL-Dithiothreitol  >98.0%(T)

  • 3483-12-3

  • 1g

  • 270.00CNY

  • Detail
  • TCI America

  • (D1071)  DL-Dithiothreitol  >98.0%(T)

  • 3483-12-3

  • 5g

  • 705.00CNY

  • Detail
  • TCI America

  • (D1071)  DL-Dithiothreitol  >98.0%(T)

  • 3483-12-3

  • 25g

  • 2,480.00CNY

  • Detail
  • Alfa Aesar

  • (A15797)  1,4-Dithio-DL-threitol, 98%   

  • 3483-12-3

  • 1g

  • 314.0CNY

  • Detail
  • Alfa Aesar

  • (A15797)  1,4-Dithio-DL-threitol, 98%   

  • 3483-12-3

  • 5g

  • 792.0CNY

  • Detail
  • Alfa Aesar

  • (A15797)  1,4-Dithio-DL-threitol, 98%   

  • 3483-12-3

  • 25g

  • 2984.0CNY

  • Detail
  • Sigma

  • (646563)  DL-Dithiothreitolsolution  1 M in H2O

  • 3483-12-3

  • 646563-10X.5ML

  • 731.25CNY

  • Detail
  • Sigma

  • (43819)  DL-Dithiothreitol  ≥99.0% (RT)

  • 3483-12-3

  • 43819-1G

  • 568.62CNY

  • Detail
  • Sigma

  • (43819)  DL-Dithiothreitol  ≥99.0% (RT)

  • 3483-12-3

  • 43819-5G

  • 1,691.82CNY

  • Detail
  • Sigma

  • (43819)  DL-Dithiothreitol  ≥99.0% (RT)

  • 3483-12-3

  • 43819-25G

  • 6,323.85CNY

  • Detail
  • Sigma-Aldrich

  • (D0632)  DL-Dithiothreitol  ≥98% (TLC), ≥99.0% (titration)

  • 3483-12-3

  • D0632-1G

  • 257.40CNY

  • Detail
  • Sigma-Aldrich

  • (D0632)  DL-Dithiothreitol  ≥98% (TLC), ≥99.0% (titration)

  • 3483-12-3

  • D0632-5G

  • 527.67CNY

  • Detail

3483-12-3SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 11, 2017

Revision Date: Aug 11, 2017

1.Identification

1.1 GHS Product identifier

Product name 1,4-dithiothreitol

1.2 Other means of identification

Product number -
Other names DL-1,4-Dithiothreitol

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:3483-12-3 SDS

3483-12-3Relevant articles and documents

Charge Accumulation and Multi-Electron Photoredox Chemistry with a Sensitizer–Catalyst–Sensitizer Triad

Nomrowski, Julia,Guo, Xingwei,Wenger, Oliver S.

, p. 14084 - 14087 (2018/09/11)

Photoinduced electron transfer in donor–sensitizer–acceptor compounds usually leads to simple electron–hole pairs, and photoredox catalysis typically relies on single-electron transfer (SET) events. This work reports on a molecular triad able to accumulate two electrons on a central dibenzo[1,2]dithiin moiety flanked by two peripheral RuII photosensitizers. Under continuous illumination, the doubly reduced form of the dibenzo[1,2]dithiin undergoes thiolate–disulfide exchange with an aliphatic disulfide substrate, thereby acting as a two-electron catalyst after two initial SET events with triethylamine at the RuII sensitizers. The use of a relatively simple triad for coupling two separate SET processes to a subsequent two-electron reduction is an important conceptual advance from photoinduced SET and light-driven charge accumulation towards multi-electron photoredox catalysis. This is relevant for artificial photosynthesis and light-driven multi-electron chemistry in general.

4-hydroxyquinoline-3-carboxamides and hydrazides as antiviral agents

-

, (2008/06/13)

The present invention provides 4-hydroxyquinoline-3-carboxamide and hydrazide compounds of formula I These compounds are useful to treat or prevent the herpesviral infections, particularly, human cytomegaloviral infection.

BIOLOGICALLY ACTIVE COMPOUNDS ISOLATED FROM AEROBIC FERMENTATION OF TRICHODERMA VIRIDE

-

, (2008/06/13)

This invention relates to compounds of structural formula (I) isolated from an aerobic fermentation of Trichoderma viride MF5628, ATCC 74084: (I) which are squalene synthase inhibitors and thus useful as cholesterol lowering agents. These compounds are also potent antifungal agents. Additionally, they inhibit farnesyl protein transferase and farnesylation of the oncogene protein Ras and are thus useful in treating cancer. This invention also relates to a process for obtaining compounds of structural formula (I)

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