51306-35-5

Basic information

• Product Name: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE
• Synonyms: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE;5-(4,6-DICHLOROTRIAZINYL)AMINOFLUORESCEIN;5-DTAF;DTAF 2HCL;DTAF HCL;5-((4,6-dichloro-1,3,5-triazin-2-yl)amino)fluorescein;5-(4,6-dichloro-S-triazin-2-ylamino) fluorescein-hcl;4-(4,6-dichloro-s-triazin-2-ylamino)fluorescein 2HCl
• CAS NO: 51306-35-5
• Molecular Formula: C₂₃H₁₂Cl₂N₄O₅
• Molecular Weight: 568.19
• Mol File: 51306-35-5.mol

Chemical Properties

• Melting Point: >325°C
• Boiling Point: 834.4 °C at 760 mmHg
• Flash Point: 458.4 °C
• Appearance: /
• Density: 1.81g/cm³
• Vapor Pressure: 4.24E-29mmHg at 25°C
• Storage Temp.: 2-8°C
• PKA: 9.33±0.20(Predicted)
• CAS DataBase Reference: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE(CAS DataBase Reference)
• NIST Chemistry Reference: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE(51306-35-5)
• EPA Substance Registry System: 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE(51306-35-5)

Safety Data

• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 51306-35-5(Hazardous Substances Data)

Usage

Uses

Used in Research and Diagnostic Applications:
5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE is used as a fluorescent reagent for the conjugation of fluorescein to proteins, carbohydrates, and selected polymers. This application is particularly useful in the field of life sciences, where it aids in the visualization, detection, and quantification of biomolecules, enabling researchers to study their structure, function, and interactions in detail.
Used in Biotechnology Industry:
In the biotechnology industry, 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE is used as a labeling agent for various biological molecules. The strong fluorescence of 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE allows for the easy tracking and detection of labeled molecules in a variety of assays, such as enzyme-linked immunosorbent assays (ELISA), flow cytometry, and fluorescence microscopy. This enhances the efficiency and accuracy of these techniques, leading to improved research outcomes and diagnostic capabilities.
Used in Pharmaceutical Industry:
5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE is also utilized in the pharmaceutical industry for the development of drug candidates and the study of drug-biomarker interactions. 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE's fluorescent properties enable researchers to monitor the distribution, metabolism, and excretion of drugs in the body, providing valuable insights into their pharmacokinetic and pharmacodynamic properties. This information is crucial for the optimization of drug formulations and the development of more effective therapeutic strategies.
Used in Environmental Monitoring:
In the field of environmental science, 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE is employed as a tracer compound for monitoring the presence and movement of pollutants in aquatic ecosystems. 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE's strong fluorescence allows for the sensitive detection of even trace amounts of contaminants, enabling researchers to assess the health of aquatic environments and develop strategies for pollution control and remediation.
Used in Material Science:
5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE is also used in material science for the development of advanced fluorescent materials and sensors. 5-(4,6-DICHLORO-S-TRIAZIN-2-YLAMINO)FLUORESCEIN-HYDROCHLORIDE's unique optical properties make it an attractive candidate for the creation of novel materials with enhanced sensing capabilities, such as those used in chemical and biological detection systems, as well as in the development of new imaging technologies.

InChI:InChI=1/C23H12Cl2N4O5.ClH/c24-20-27-21(25)29-22(28-20)26-10-1-4-14-13(7-10)19(32)34-23(14)15-5-2-11(30)8-17(15)33-18-9-12(31)3-6-16(18)23;/h1-9,30-31H,(H,26,27,28,29);1H

Relevant articles and documents

Single label comparative hybridization

The present invention provides methods of detecting and mapping chromosomal or genetic abnormalities associated with various diseases or with predisposition to various diseases, or to detecting the phenomena of large scale copy number variants. In particular, the present invention provides advanced methods of performing array-based comparative hybridization that allow reproducibility between samples and enhanced sensitivity by using the same detectable label for both test sample and reference sample nucleic acids. Invention methods are useful for the detection or diagnosis of particular disease conditions such as cancer, and detecting predisposition to cancer based on detection of chromosomal or genetic abnormalities and gene expression level. Invention methods are also useful for the detection or diagnosis of hereditary genetic disorders or predisposition thereto, especially in prenatal samples. Moreover, invention methods are also useful for the detection or diagnosis of de novo genetic aberrations associated with post-natal developmental abnormalities.

Nucleic acid size detection method

The present invention provides methods of determining the size of a particular nucleic acid segment of interest in a sample of nucleic acids through fragmentation of DNA, size fractionation, an optional second fragmentation, and identification using a marker sequence. In particular aspects, an expansion or reduction of tandem repeat sequences can be detected. In further aspects, carriers and individuals afflicted with fragile X syndrome or other diseases associated with tandem repeats can be distinguished from normal individuals.

Assay for mycobacterium avium/intracellulare nucleic acid

Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotides.

Substractive single label comparative hybridization

Provided are methods of determining differences between nucleic acids in a test sample and a reference sample. In certain embodiments the methods are used for detecting and mapping chromosomal or genetic abnormalities associated with various diseases or with predisposition to various diseases, or to detecting the phenomena of large scale copy number variants. In particular, provided are advanced methods of performing array-based comparative hybridization that allow reproducibility between samples and enhanced sensitivity by using the same detectable label for both test sample and reference sample nucleic acids. Invention methods are useful for the detection or diagnosis of particular disease conditions such as cancer, and detecting predisposition to cancer based on detection of chromosomal or genetic abnormalities and gene expression level. Invention methods are also useful for the detection or diagnosis of hereditary genetic disorders or predisposition thereto, especially in prenatal samples. Moreover, invention methods are also useful for the detection or diagnosis of de novo genetic aberrations associated with post-natal developmental abnormalities.

Activatable probes and methods for in vivo gene detection

Probes for detecting a target polynucleotide are provided. One aspect provides a molecular beacon probe set wherein the donor molecular beacon comprises a quantum dot and an acceptor molecular beacon comprises at least one reporter. The probes optionally comprise a protein transduction domain, targeting signal, or a combination thereof. Methods for detecting target polynucleotides using the disclosed probes are also provided.

Oligonucleotides comprising a molecular switch

This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.

Dual resonance energy transfer nucleic acid probes

Dual nucleic acid probes with resonance energy transfer moieties are provided. In particular, fluorescent or luminescent resonance energy transfer moieties are provided on hairpin stem-loop molecular beacon probes that hybridize sufficiently near each other on a subject nucleic acid, e.g. mRNA, to generate an observable interaction. The invention also provides lanthanide chelate luminescent resonance energy transfer moieties on linear and stem-loop probes that hybridize sufficiently near each other on a subject nucleic acid to generate an observable interaction. The invention thereby provides detectable signals for rapid, specific and sensitive hybridization determination in vivo. The probes are used in methods of detection of nucleic acid target hybridization for the identification and quantification of tissue and cell-specific gene expression levels, including response to external stimuli, such as drug candidates, and genetic variations associated with disease, such as cancer.

Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This "closed-tube" format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.