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Stereoselective synthesis of L-[15N] amino acids with glucose dehydrogenase and galactose mutarotase as NADH regenerating system
Chiriac, Maria,Lupan, Iulia,Bucurenci,Popescu,Palibroda
, p. 171 - 174 (2008/09/19)
We have developed an efficient stereospecific enzymatic synthesis of L-[15N]-valine, L-[15N]-leucine, L-[15N]- norvaline, L-[15N]-norleucine and L-[15N]-isoleucine from the corresponding α-keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L-amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale (1-10 g). Copyright
Chemo-Enzymatic Syntheses of Isotopically Labelled L-Amino Acids
Kelly, Nicholas M.,O'Neill, Bridget C.,Probert, John,Reid, Gordon,Stephen, Rosamund,et al.
, p. 6533 - 6536 (2007/10/02)
L-Alanine labelled with carbon-13 and nitrogen-15 has been prepared in good yield and high optical purity from achiral precursors using alanine dehydrogenase.This versatile approach may be simply adapted for the preparation of a range of isotopically labe