615-28-1 Usage
Uses
Different sources of media describe the Uses of 615-28-1 differently. You can refer to the following data:
1. o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
2. o-Phenylenediamine dihydrochloride has been used as a substrate for horseradish-peroxidase-conjugated secondary antibody in enzyme-linked immunosorbent assay (ELISA).
General Description
Brownish-yellow crystals. Slightly soluble in water. Used in the manufacture of dye.
Air & Water Reactions
Slightly soluble in water.
Reactivity Profile
Acidic organic/inorganic salts, such as OPD EASY-tablets, are generally soluble in water. The resulting solutions contain moderate to high concentrations of hydrogen ions and have pH's of less than 7.0. They react as acids to neutralize bases. These neutralizations generate heat, but less or far less than is generated by neutralization of inorganic acids, inorganic oxoacids, and carboxylic acid. They usually do not react as either oxidizing agents or reducing agents but such behavior is not impossible. Many of these compounds catalyze organic reactions.
Purification Methods
Crystallise the salt from dilute HCl (60mL conc HCl, 40mL water, with 2g stannous chloride), after treatment of the hot solution with charcoal by adding an equal volume of conc HCl and cooling in an ice-salt mixture. The crystals are washed with a small amount of conc HCl and dried in a vacuum desiccator over NaOH. [Beilstein 13 IV 38.]
Check Digit Verification of cas no
The CAS Registry Mumber 615-28-1 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 6,1 and 5 respectively; the second part has 2 digits, 2 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 615-28:
(5*6)+(4*1)+(3*5)+(2*2)+(1*8)=61
61 % 10 = 1
So 615-28-1 is a valid CAS Registry Number.
InChI:InChI=1/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
615-28-1Relevant articles and documents
Reduction of dinitrobenzenes by electron-carrying catalysts in the electrosynthesis of diaminobenzenes
Abakumov, M. V.,Leonova, M. Yu.,Mikhalchenko, L. V.,Novikov, V. T.,Zaplavin, A. P.
, p. 1927 - 1933 (2021/11/05)
The interaction of isomeric dinitrobenzenes (DNBs) with titanium(III), tin(II), and vanadium(II) chlorides, which are reducing agents used as electron carriers in the electrosynthesis of diaminobenzenes, has been studied. Rate constants of the reduction of isomeric DNBs and nitrophenylhydroxylamines by SnCl2 and TiCl3 in a 2 M water-alcohol solution (10 vol.% C2H5OH) of HCl were measured, and activation energies of the reduction of isomeric DNBs were determined. The rates of interaction of DNBs with the listed mediators increase in the series SnCl2 3 2. It is shown that the electrolysis of DNBs in the presence of an excess of these mediators makes it possible to obtain the corresponding diaminobenzenes with a yield of 60–90%.
