64285-73-0

Basic information

• Product Name: 3,3',5,5'-Tetramethylbenzidine dihydrochloride
• Synonyms: 3,3',5,5'-TETRAMETHYL-[1,1'-BIPHENYL]-4,4'-DIAMINE, DIHYDROCHLORIDE;3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE MONOHYDRATE;3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE;3,3',5,5'-TETRAMETHYLBENZIDINE 2HCL;4,4'-DIAMINO-3,3',5,5'-TETRAMETHYLBIPHENYL DIHYDROCHLORIDE;4,4'-DIAMINO-3,3',5,5'-TETRAMETHYLBIPHENYL DIHYDROCHLORIDE MONOHYDRATE;DIHYDROCHLORIDE TMB;TMB-HCL
• CAS NO: 64285-73-0
• Molecular Formula: C16H22Cl2N2
• Molecular Weight: 313.27
• EINECS: 264-769-6
• Product Categories: Biphenyl & Diphenyl ether;Enzyme substrates
• Mol File: 64285-73-0.mol

Chemical Properties

• Melting Point: ≥300 °C
• Boiling Point: 368.6 °C at 760 mmHg
• Flash Point: 54 °C
• Appearance: /tablet
• Storage Temp.: 2-8°C
• Sensitive: Moisture & Light Sensitive
• CAS DataBase Reference: 3,3',5,5'-Tetramethylbenzidine dihydrochloride(CAS DataBase Reference)
• NIST Chemistry Reference: 3,3',5,5'-Tetramethylbenzidine dihydrochloride(64285-73-0)
• EPA Substance Registry System: 3,3',5,5'-Tetramethylbenzidine dihydrochloride(64285-73-0)

Safety Data

• Hazard Codes: Xn,T
• Statements: 40-39/23/24/25-23/24/25-10
• Safety Statements: 22-24/25-36/37-45
• WGK Germany: 3
• F: 3-10-23
• TSCA: Yes
• Hazardous Substances Data: 64285-73-0(Hazardous Substances Data)

Usage

Uses

Used in Enzyme-Linked Immunosorbent Assay (ELISA):
3,3',5,5'-Tetramethylbenzidine dihydrochloride is used as a peroxidase substrate for ELISA procedures, enabling the detection and quantification of specific antigens or antibodies in a sample.
Used in Anti-mouse IgG Horseradish Peroxidase-Conjugated Antibody Assay:
In the ELISA of mice sera, 3,3',5,5'-Tetramethylbenzidine dihydrochloride is used as a substrate for the anti-mouse IgG horseradish peroxidase-conjugated antibody, aiding in the identification and measurement of specific immunoglobulins in mouse serum.
Used in Anti-mouse Peroxidase Antibody Assay:
3,3',5,5'-Tetramethylbenzidine dihydrochloride is employed as a substrate in the anti-mouse peroxidase antibody assay, which is utilized in the analysis of protein tyrosine phosphatase in naive and effector T cell lysates.
Used in Enzymatic Hydroperoxide (EH) Assay:
3,3',5,5'-Tetramethylbenzidine dihydrochloride is used as a substrate in the enzymatic hydroperoxide assay, which is a method for evaluating the activity of enzymes involved in the metabolism of hydrogen peroxide and other peroxides.
Used in Prooxidants-Antioxidants Balance (PAB) Assay:
3,3',5,5'-Tetramethylbenzidine dihydrochloride is utilized as a substrate in the prooxidants-antioxidants balance assay of serum samples from diabetic patients, helping to assess the balance between prooxidant and antioxidant levels in the blood.