75621-03-3 Usage
Uses
Used in Laboratory Research:
CHAPS is used as a non-denaturing solvent for dissolving biological macromolecules, particularly proteins. Its zwitterionic nature allows it to interact gently with proteins, preventing denaturation and preserving their native structure, which is crucial for various laboratory applications such as protein purification and analysis.
Used in Membrane Protein Purification:
In the field of membrane biochemistry, CHAPS is utilized as a non-denaturing detergent for the purification of membrane proteins. Its ability to dissolve these proteins without disrupting their structure makes it an invaluable tool for studying the properties and functions of membrane proteins, which are often challenging to work with due to their hydrophobic nature.
Used in Combination with Nonionic Detergents:
CHAPS is also useful in conjunction with nonionic detergents like Triton X-100. This combination can enhance the solubility of proteins and improve the efficiency of purification processes, making it a versatile choice for researchers working with a variety of protein types and systems.
Biological Activity
CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. Combines the properties of both the sulfobetaine-type and bile-acid detergents.
Check Digit Verification of cas no
The CAS Registry Mumber 75621-03-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,5,6,2 and 1 respectively; the second part has 2 digits, 0 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 75621-03:
(7*7)+(6*5)+(5*6)+(4*2)+(3*1)+(2*0)+(1*3)=123
123 % 10 = 3
So 75621-03-3 is a valid CAS Registry Number.
InChI:InChI=1/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
75621-03-3Relevant articles and documents
A Switchable Open/closed Polyaromatic Macrocycle that Shows Reversible Binding of Long Hydrophilic Molecules
Kurihara, Kohei,Yazaki, Kohei,Akita, Munetaka,Yoshizawa, Michito
, p. 11360 - 11364 (2017)
In spite of wide-ranging previous studies on synthetic macrocycles, the installation of open–close functions into the frameworks remains a challenge. We present a new polyaromatic macrocycle capable of switching between open and closed forms in response to external stimuli, namely, base and acid. The macrocycle, which is prepared in three steps, has a well-defined hydrophobic cavity with a length of around 1 nm, surrounded by four pH-responsive acridinium panels. The open and closed structures were confirmed by single-crystal X-ray analysis. The cylindrical cavity can bind long hydrophilic molecules up to 2.7 nm in length in neutral water and then release the bound guests through a reversible open-to-closed structural change upon simple addition of base.
