7612-98-8

Basic information

• Product Name: 4-(N,N-DIMETHYLAMINO)AZOBENZENE-4'-ISOTHIOCYANATE
• Synonyms: DABITC;4-(4-ISOTHIOCYANATOPHENYLAZO)-N,N-DIMETHYLANILINE;4-DIMETHYLAMINOAZOBENZENE 4'-ISOTHIOCYANATE;4-(N,N-DIMETHYLAMINO)AZOBENZENE-4'-ISOTHIOCYANATE;4-N,N-DIMETHYLAMINOAZOBENZENE-4-ISOTHIOCYANATE;4-((4-ISOTHIOCYANATOPHENYL)AZO)-N N- &;Benzenamine, 4-(4-isothiocyanatophenyl)azo-N,N-dimethyl-;4-(Dimethylamino)azobenzene-4μ-isothiocyanate, DABITC
• CAS NO: 7612-98-8
• Molecular Formula: C₁₅H₁₄N₄S
• Molecular Weight: 282.36
• EINECS: 231-521-3
• Product Categories: marker
• Mol File: 7612-98-8.mol
• Article Data: 3

Chemical Properties

• Melting Point: 167-171 °C(lit.)
• Boiling Point: 466.2°C at 760 mmHg
• Flash Point: 235.7°C
• Appearance: /
• Density: 1.2493 (rough estimate)
• Vapor Pressure: 7.25E-09mmHg at 25°C
• Refractive Index: 1.5700 (estimate)
• Solubility: dioxane: 20 mg/mL, clear, red
• PKA: 3.28±0.10(Predicted)
• Sensitive: Moisture Sensitive
• BRN: 752568
• CAS DataBase Reference: 4-(N,N-DIMETHYLAMINO)AZOBENZENE-4'-ISOTHIOCYANATE(CAS DataBase Reference)
• NIST Chemistry Reference: 4-(N,N-DIMETHYLAMINO)AZOBENZENE-4'-ISOTHIOCYANATE(7612-98-8)
• EPA Substance Registry System: 4-(N,N-DIMETHYLAMINO)AZOBENZENE-4'-ISOTHIOCYANATE(7612-98-8)

Safety Data

• Hazard Codes: Xn
• Statements: 42
• Safety Statements: 22-24/25
• RIDADR: 3234
• WGK Germany: 3
• RTECS: NX9125000
• F: 10-21
• HazardClass: IRRITANT
• Hazardous Substances Data: 7612-98-8(Hazardous Substances Data)

Usage

Chemical Properties

Rust crystalline powder

Uses

4-(4-Isothiocyanatophenylazo)-N,N-dimethylaniline is a stain/dye. Dyes and metabolites.

Purification Methods

Crystallise DABITC by dissolving 1g in 150mL of boiling Me2CO, filtering hot and allowing to cool at -20o overnight, collecting the solid and drying it in a vacuum. Solutions in pyridine should be used immediately, otherwise it decomposes. It is moisture sensitive. [Chang Methods Enzymol 91 79, 455 1983.]

InChI:InChI=1/C15H14N4S/c1-19(2)15-9-7-14(8-10-15)18-17-13-5-3-12(4-6-13)16-11-20/h3-10H,1-2H3/b18-17+

Relevant articles and documents

High sensitivity sequence analysis of peptides and proteins by 4 NN dimethylaminoazobenzene 4' isothiocyanate

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Dual resonance energy transfer nucleic acid probes

Dual nucleic acid probes with resonance energy transfer moieties are provided. In particular, fluorescent or luminescent resonance energy transfer moieties are provided on hairpin stem-loop molecular beacon probes that hybridize sufficiently near each other on a subject nucleic acid, e.g. mRNA, to generate an observable interaction. The invention also provides lanthanide chelate luminescent resonance energy transfer moieties on linear and stem-loop probes that hybridize sufficiently near each other on a subject nucleic acid to generate an observable interaction. The invention thereby provides detectable signals for rapid, specific and sensitive hybridization determination in vivo. The probes are used in methods of detection of nucleic acid target hybridization for the identification and quantification of tissue and cell-specific gene expression levels, including response to external stimuli, such as drug candidates, and genetic variations associated with disease, such as cancer.