909729-58-4Relevant articles and documents
Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics
de Geus, Mark A. R.,Maurits, Elmer,Sarris, Alexi J. C.,Hansen, Thomas,Kloet, Max S.,Kamphorst, Kiki,ten Hoeve, Wolter,Robillard, Marc S.,Pannwitz, Andrea,Bonnet, Sylvestre A.,Codée, Jeroen D. C.,Filippov, Dmitri V.,Overkleeft, Herman S.,van Kasteren, Sander I.
supporting information, p. 9900 - 9904 (2020/06/05)
The inverse electron demand Diels–Alder pyridazine elimination reaction between tetrazines and allylic substituted trans-cyclooctenes (TCOs) is a key player in bioorthogonal bond cleavage reactions. Determining the rate of elimination of alkylamine substrates has so far proven difficult. Here, we report a fluorogenic tool consisting of a TCO-linked EDANS fluorophore and a DABCYL quencher for accurate determination of both the click and release rate constants for any tetrazine at physiologically relevant concentrations.
Quenched ligand-directed tosylate reagents for one-step construction of turn-on fluorescent biosensors
Tsukiji, Shinya,Wang, Hangxiang,Miyagawa, Masayoshi,Tamura, Tomonori,Takaoka, Yousuke,Hamachi, Itaru
supporting information; experimental part, p. 9046 - 9054 (2009/12/04)
Semisynthetic fluorescent biosensors consisting of a protein framework and a synthetic fluorophore are powerful analytical tools for specific detection of biologically relevant molecules. We report herein a novel method that allows for the construction of
