95233-18-4 Usage
Antimalarial Agent
The hydroxynaphthoquinone atovaquone, which exhibits antimalarial and anti-Pneumocystis activity, is an electron transport inhibitor that causes depletion of the ATP pool. The primary effect is on the iron flavoprotein dihydro-orotate dehydrogenase, an essential enzyme in the production of pyrimidines. Mammalian cells are able to avoid undue toxicity by use of preformed pyrimidines. Dihydro-orotate dehydrogenase from Plasmodium falciparum is inhibited by concentrations of atovaquone that are very much lower than those needed to inhibit the Pneumocystis enzyme, raising the possibility that the antimicrobial consequences might differ in the two organisms. Although atovaquone was originally developed as a monotherapy for malaria, high level resistance readily emerges in Plasmodium falciparum when the drug is used alone. Consequently, atovaquone is now combined with proguanil.
Description
Atovaquone is an orally active antiprotozoal agent indicated for patients with mild to
moderate AIDS-associated Pneumocystis carinii pneumonia who are intolerant to the
fist-line therapy of trimethoprim-sulfamethoxazole. It is also under investigation as a
treatment for malaria and AIDS-associated toxoplasmosis.
Uses
Atovaquone inhibits the cytochrome bc(1) complex via interactions with the Rieske iron-sulfur protein and cytochrome b in the ubiquinol oxidation pocket. In addition to its use as a treatment for toxoplasmosis, atovaquone has antimalarial properties and prevents pneumocystis pneumonia post-renal transplant.
Definition
ChEBI: A naphthoquinone compound having a 4-(4-chlorophenyl)cyclohexyl group at the 2-position and a hydroxy substituent at the 3-position.
Indications
Atovaquone is a naphthoquinone whose mechanism of
action involves inhibition of the mitochondrial electron
transport system in the protozoa. Malaria parasites depend
on de novo pyrimidine biosynthesis through dihydroorotate
dehydrogenase coupled to electron transport.
Plasmodia are unable to salvage and recycle
pyrimidines as do mammalian cells.
Atovaquone is poorly absorbed from the gastrointestinal
tract, but absorption is increased with a fatty
meal. Excretion of the drug, mostly unchanged, occurs
in the feces.The elimination half-life is 2 to 3 days. Low
plasma levels persist for several weeks. Concurrent administration
of metoclopramide, tetracycline, or rifampin
reduces atovaquone plasma levels by 40 to 50%.
Atovaquone has good initial activity against the
blood but not the hepatic stage of P. vivax and P. ovale
malaria parasites. It is effective against erythrocytic and
exoerythrocytic P. falciparum, and therefore, daily suppressive
doses need to be taken for only 1 week upon
leaving endemic areas.When used alone, it has an unacceptable
(30%) rate of recrudescence and selects for resistant
organisms. It and proguanil are synergistic when
combined and no atovaquone resistance is seen. This
combination (Malarone) is significantly more effective
than mefloquine, amodiaquine, chloroquine, and combinations
of chloroquine, pyrimethamine, and sulfadoxine.
In addition to using the combination of atovaquone
and proguanil for the treatment and prophylaxis of P.
falciparum malaria, atovaquone is also used for the
treatment and prevention of P. carinii pneumonia and
babesiosis therapy.
Atovaquone is well tolerated and produces only
rare instances of nausea, vomiting, diarrhea, abdominal
pain, headache, and rash of mild to moderate intensity.
Manufacturing Process
Preparation of intermediate 4-(4-chlorophenyl)cyclohexane-1-carboxylic acid
was needed at first. It was made as follows: acetyl chloride (30 g), aluminium
chloride (60 g) in carbon disulfide (120 ml) were stirred at -50°C. Cyclohexen
(30 g) previously cooled to -50°C was added dropwise during 10 minutes and
the mixture was stirred for 60 minutes at -50°C. The solvent was decanted
and 300 ml chlorobenzene was added, the so-obtained solution heated at
40°C for 3 hours with stirring, poured onto a mixture of ice and concentrated
hydrochloric acid and the organic layer washed with 2 M HCl, 2 M NaOH and
water, dried over anhydrous Na2SO4. The product was distilled in vacuo, the
fraction boiling at 140°-154°C (0.1 mm Hg) collected, diluted with an equal
volume of petroleum ether, cooled to -6°C and a stream of nitrogen gas
bubbled through.
3.1 g above obtained hexahydroacetophenone was dissolved in dioxan (15 ml)
and the fresh preparated hypobromite (8 ml) in a solution of NaOH (6.2 g) in
water (42 ml) at 0°C was added at below 20°C. The mixture was stirred at
ambient temperature for 6 hours then allowed to stand overnight. Excess
hypobromite was destroyed with sodium metabisulphite, cooled and then
acidified to give a colourless solid. It was filtered off, washed with water, dried
and recrystallysed from ethanol to give 4-(4-chlorophenyl)cyclohexane-1-
carboxylic acid, m.p. 254°-256°C. A mixture of this acid, 2-chloro-1,4-
naphthoquinone and silver nitrate was added to ammonium persulfate to give
the corresponding naphthoquinone which was saponificated with KOH and was
yielded 2-trans-4-(p-chlorophenyl)cyclohexyl)-3-hydroxy-1,4 naphthoquinone,
m.p. 216°-219°C, shown by NMR to be the pure trans isomer.
Brand name
Mepron (GlaxoSmithKline).
Antimicrobial activity
It is active against erythrocytic, liver and sexual stages of
malaria parasites. It shows synergy with proguanil and tetracyclines
in vitro. It is also active against Babesia spp. and both
tachyzoites and cysts of Tox. gondii. Pn. jirovecii is sensitive in
vitro at 0.1–3.0 mg/L and high doses are effective in the rat.
Acquired resistance
Point mutations on parasite cytochrome b, in particular
at codon 268, cause resistance and readily occur when the
drug is used alone. The rapid selection of resistance led to
the development of the synergistic combination with proguanil.
Failure of Pn. jirovecii prophylaxis has also been associated
with cytochrome b mutations.
General Description
Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.Atovaquone belongs to the class of naphthoquinones with a broad-spectrum activity against parasitic infections including malaria, toxoplasmosis and Pneumocystis pneumonia. It is a potent antiprotozoal compound which aids in collapsing the mitochondrial membrane potential in a malaria parasite.
Pharmaceutical Applications
A hydroxynaphthoquinone. Available as the trans isomer
(which is more active than the cis form) for oral use. It is insoluble
in water.
Biochem/physiol Actions
Atovaquone is an anti-protozoal mitochondrial electron transport inhibitor; Antimalarial; Antipneumocystic, and has also been used to treat toxoplasmosis. It is an analog of protozoan mitochondrial protein ubiquinone, and acts by inhibiting the cytochrome bc(1) complex via interactions with the Rieske iron-sulfur protein and cytochrome b in the ubiquinol oxidation pocket.
Mechanism of action
Atovaquone is thought to produce its antiparasitic action by virtue of its ability to
inhibit the mitochondrial respiratory chain. More specifically, atovaquone is a ubiquinone
reductase inhibitor, inhibiting at the cytochrome bc1 complex. This action leads to a collapse
of the mitochondrial membrane potential. The compound shows stereospecific inhibition, with the
trans isomer being more active than the cisisomer.
Pharmacokinetics
Oral absorption: Poor
Cmax 750 mg oral: 27 mg/L (steady state)
Plasma half-life: 70 h
Plasma protein binding: >99%
It is highly lipophilic and is poorly absorbed from the gastrointestinal
tract following oral administration. Bioavailability is
improved when administered with meals, particularly those
with a high fat content. Steady-state plasma concentrations
are up to 50% lower in AIDS patients than in asymptomatic
HIV-positive cases and the elimination half-life is lower (55 h)
in patients with AIDS. The concentration in CSF is <1% of
the plasma level. Unlike some other naphthoquinones it is not
metabolized by human liver microsomes. Combinations with
co-trimoxazole (in HIV patients) and with proguanil plus
artesunate in healthy adults did not produce any changes in
atovaquone pharmacokinetics.
Clinical Use
Different sources of media describe the Clinical Use of 95233-18-4 differently. You can refer to the following data:
1. 3-[4-(4-Chlorophenyl)-cyclohexyl]-2-hydroxy-1,4-naphthoquinone(Mepron) is a highly lipophilic, water-insolubleanalog of ubiquinone 6, an essential component of the mitochondrialelectron transport chain in microorganisms. Thestructural similarity between atovaquone and ubiquinonesuggests that the former may act as an antimetabolite for thelatter and thereby interfere with the function of electrontransport enzymes.Atovaquone was originally developed as an antimalarialdrug, but Plasmodium falciparum was found to developa rapid tolerance to its action. More recently, the effectivenessof atovaquone against P. carinii was discovered. Itis a currently recommended alternative to trimethoprimsulfamethoxazole(TMP-SMX) for the treatment and prophylaxisof PCP in patients intolerant to this combination.Atovaquone was also shown to be effective in eradicatingT. gondii in preclinical animal studies.The oral absorption of atovaquone is slow and incomplete,in part because of the low water solubility of the drug.Aqueous suspensions provide significantly better absorptionthan do tablets. Food, especially if it has a high fat content,increases atovaquone absorption. Significant enterohepaticrecycling of atovaquone occurs, and most (nearly 95%) ofthe drug is excreted unchanged in the feces. In vivo, atovaquoneis largely confined to the plasma, where it is extensivelyprotein bound ( 99.9%). The half-life of the drugranges from 62 to 80 hours. The primary side effect is gastrointestinalintolerance.
2. Pn. jirovecii pneumonia; alternative therapy for mild to moderate illness
(prophylaxis and treatment)
Prophylaxis and treatment of malaria in combination with proguanil
It has also been used in cerebral toxoplasmosis in AIDS
patients and in a few cases of human babesiosis.
Side effects
Most clinical trials of atovaquone alone have involved patients
with AIDS in whom adverse effects are often difficult to
detect; however, more than 20% reported fever, nausea, diarrhea
and rashes. There were limited changes in hepatocellular
function. In malaria, in combination with proguanil, there are
few reported side effects.
Veterinary Drugs and Treatments
Atovaquone (with azithromycin) appears effective in treating dogs
with Babesia gibsoni (Asian genotype) infections, particularly in
dogs not immunosuppressed or splenectomized. Atovaquone may
be of benefit for treating pneumocystosis in dogs, but it is considered
second line therapy after potentiated sulfonamides.
Atovaquone (with azithromycin) may be of benefit in treating
Cytauxzoon felis infections in cats (research is in progress at the time
of writing).
Drug interactions
Potentially hazardous interactions with other drugs
Antibacterials: avoid with rifabutin, concentration
of both drugs reduced; avoid with rifampicin,
concentration reduced and rifampicin concentration
increased; concentration reduced by tetracycline.
Antivirals: concentration reduced by efavirenz -
avoid; concentration of indinavir possibly reduced;
concentration of zidovudine increased.
Metoclopramide: significant reduction in plasma
atovaquone levels.
Metabolism
Atovaquone is poorly absorbed from the GI tract because of its poor water solubility and high fat
solubility, but the absorption can be significantly increased if taken with a fat-rich meal. The drug
is highly bound to plasma protein (94%) and does not enter the CNS in significant quantities. It is
not significantly metabolized in humans and is exclusively eliminated in feces via the bile.
Check Digit Verification of cas no
The CAS Registry Mumber 95233-18-4 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,5,2,3 and 3 respectively; the second part has 2 digits, 1 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 95233-18:
(7*9)+(6*5)+(5*2)+(4*3)+(3*3)+(2*1)+(1*8)=134
134 % 10 = 4
So 95233-18-4 is a valid CAS Registry Number.
InChI:InChI=1/C22H19ClO3/c23-16-11-9-14(10-12-16)13-5-7-15(8-6-13)19-20(24)17-3-1-2-4-18(17)21(25)22(19)26/h1-4,9-13,15,26H,5-8H2
95233-18-4Relevant articles and documents
Atovaquone preparation method
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, (2020/02/10)
The invention provides an atovaquone preparation method, which comprises: carrying out condensation, hydrolysis and decarboxylation under the action of an alkali by using o-phthalic acid diester and 2-trans-[4-(4-chlorphenyl)]cyclohexyl succinic acid diester as raw materials to obtain 2-trans-[4-(4-chlorphenyl)]cyclohexyl-2,3-dihydro-1,4-naphthalenedione, carrying out a halogen substitution reaction on the 2-trans-[4-(4-chlorphenyl)]cyclohexyl-2,3-dihydro-1,4-naphthalenedione and a halogenating reagent to obtain a dihalogenated compound mixture, eliminating hydrogen halide to obtain 2-trans-[4-(4-chlorphenyl)]cyclohexyl-3-halo-1,4-naphthalenedione, and finally hydrolyzing to obtain atovaquone (I). According to the invention, the method has advantages of cheap and easily available raw materials, safe and simple process operation, low cost, little wastewater generation, safety, environmental protection, easily achieved reaction conditions, high reaction activity, high selectivity and fewside reactions, and the prepared atovaquone is few in impurity, high in purity and high in yield.
A single-pot synthesis of atovaquone: An antiparasitic drug of choice
Dike, Suneel Y.,Singh, Dharmendra,Thankachen, Byju N.,Sharma, Brajesh,Mathur, Pramil K.,Kore, Swapnil,Kumar, Ashok
, p. 618 - 625 (2014/06/09)
The present article relates to a practical, economically viable, and validated at industrial scale, single-pot synthetic route for preparation of atovaquone, one of the most versatile antiparasitic drugs of choice used for the prophylaxis and treatment of diseases such as pneumocystis, toxoplasmosis, babesiosis, coccidiosis, and malaria. However, owing to the extremely poor yields of synthesis and very high doses of treatment (due to poor bioavailability) the cost of treatment with this drug is not affordable by the patients in need, particularly in the third world countries where these diseases are most prevalent. Unlike most of the reported processes which use 2-chloronaphthoquinone and pure trans-4-chlorophenyl cyclohexane carboxylic acid, our process is based on the decarboxylative alkylation of isomeric mixture of 4-chlorophenyl cyclohexane carboxylic acid with 1,4-naphthoquinone to give 42% overall yield of atovaquone, 10 times higher than from the reported process (4%) from the innovators of this drug.
IMPROVED SYNTHESIS OF 2-(4-(4-CHLOROPHENYL) CYCLOHEX-1-ENYL) -3, 4-DIHYDRONAPHTHALEN-1 (2H)-ONE; AN INTERMEDIATE FOR ATOVAQUONE
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Page/Page column 27; 28, (2013/03/26)
A process for preparation of 2-(4-(4-chlorophenyl) cyclohex-l-enyl)-3,4-dihydronaphthalen- 1(2H)-one (V), key intermediate for synthesis of Atovaquone [I]. The process for preparation of compound(V) comprising of the steps of; i) Reaction of 2-(4-(4-chlorophenyl)-1-hydroxycyclohexyl)-3,4-dihydronaphthalen- 1(2H)-one (IV) with trifluro acetic anhydride in presence of base in organic solvent to yield compound of formula (XIa) ii) Elimination of trifluoroacetyl functionality of compound (XIa) in organic solvent and in presence of organic base to give compound of formula (V). The invention also provides a Process for preparation of compound(XIa) comprising of the steps of; i) reaction of 2-(4-(4-chlorophenyl)-l-hydroxy cyclohexyl)-3,4-dihydronaphthalen- 1(2H)-one (IV) with trifluro acetic anhydride in presence of organic base in organic solvent. A further process is provided for preparation of compound(V) from compound (XIa) comprising elimination reaction of trifluoroacetyl functionality compound (XIa) in organic solvent and in presence of organic base.
