976-85-2Relevant articles and documents
Synthesis and characterization of new 5,5′-dimethyl- and 5,5′-diphenylhydantoin-conjugated hemorphin derivatives designed as potential anticonvulsant agents
Georgieva, Stela,Peneva, Petia,Rangelov, Miroslav,Tchekalarova, Jana,Todorov, Petar,Todorova, Nadezhda
, p. 2198 - 2217 (2022/02/16)
Herein, the synthesis and characterization of some novel N-modified hybrid analogues of hemorphins containing a C-5 substituted hydantoin residue as potential anticonvulsants and for the blockade of sodium channels are presented. Their structure-property relationships are highlighted by electrochemical and Fourier transform infrared spectroscopy (FT-IR) analysis methods. The lipophilicity and molecular docking of voltage-gated sodium channels were also determined. The new series of 5,5′-dimethyl- and 5,5′-diphenylhydantoin-conjugated hemorphin derivatives were obtained as C-terminal amides via solid-phase peptide synthesis, an Fmoc-strategy using 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethylaminium tetrafluoroborate (TBTU). The anticonvulsant activity of the hybrid-peptides (0.25, 0.5 and 1 μg) was tested by maximal electroshock (MES) and 6 Hz psychomotor seizure tests using male ICR mice. None of the compounds tested showed neurotoxicity in the rotarod test. The reference drug phenytoin was used as a positive control. The most active compound Ph-5 showed 100% efficacy against the 6 Hz-induced psychomotor seizures at a dose of 1.0 μg and tonic seizures in the MES test at a lower dose of 0.5 μg. This analogue of VV-hemorphin-5 contained a 5,5′-diphenylhydantoin residue at the N-terminus and a hydrophobic Val-Val-Tyr-Pro-Trp-Thr-Gln-CONH2 amino acid sequence of the peptide molecule. The quantitative data for the 6 Hz test demonstrated that the peptide Ph-5 exhibited a median effective dose (ED50) value of 0.358 μg and PI >13.97, and ED50 of 0.25 μg and PI >20.35 in the MES test, respectively. Results from the docking study suggest that the neuropeptide Ph-5 is a potent inhibitor of sodium channels, and blockade of voltage-gated sodium channels could be the mechanism of action of the hybrid-peptide derivatives with anticonvulsant activity.
Synthesis and anticonvulsant activity of new phenytoin derivatives
Botros,Khalil, Nadia A.,Naguib, Bassem H.,El-Dash, Yara
, p. 57 - 63 (2013/03/28)
Hybrids between phenytoin and thiosemicarbazide, 1,3,4-oxadiazole, 1,3,4-thiadiazole or 1,2,4-triazole were synthesized and tested for anticonvulsant activity. Preliminary anticonvulsant screening was performed using standard maximal electroshock (MES) an
SOME EXAMPLES OF 5,5-DIPHENYLHYDANTOIN ALKYLATION
Kiec-Kononowicz, Katarzyna,Zejc, Alfred
, p. 761 - 767 (2007/10/02)
The method of N1 and N3 alkylation of 5,5-diphenylhydantoin has been described for some examples.The synthesis of only N1 alkylated compound 11 has been accomplished with ethoxycarbonyl as the N3 protecting grou