CAT Assay

Name:
CAT Assay
Detailed information:
An enzyme assay. CAT stands for chloramphenicol acetyl transferase, a bacterial enzyme which inactivates chloramphenicol by acetylating it. CAT assays are often performed to test the function of a promoter. The gene coding for CAT is linked onto a promoter (transcription control region) from another gene, and the construct is “transfected” into cultured cells. Largely supplanted by the reporter gene luciferase.