Genomic Blot

Name:
Genomic Blot
Detailed information:
A type of Southern blot specifically used to analyze a mixture of DNA fragments derived from total genomic DNA. Because genomic DNA is very complicated, when it has been digested with restriction enzymes it produces a complex set of fragments ranging from tens of bp to tens of thousands of bp. However, any specific gene will be reproducibly found on only one or a few specific fragments. A million identical cells will produce a million identical restriction fragments for any given gene, so probing a genomic Southern with a gene-specific probe will produce a pattern of perhaps one or just a few bands.