The chemical transformations occurring in an organism from the time a nutrient substance enters it until it has been utilized and the waste products eliminated. In animals and humans, digestion and absorption are primary steps, followed by a complicated series of degradations, syntheses, hydrolyses, and oxidations, in which agents such as enzymes, bile acids, and hydrochloric acid take part. These transformations are often localized with respect to organs, tissues, and types of cells involved. Basal metabolism is the rate of total heat production of an individual who is awake but in complete mental and physical repose, at comfortable temperature and without having had food for at least 12 hrs. Under these conditions, oxidation of stored nutrients provides the sole source of energy expended and heat is measurable by calorimetry. See Digestion.