Stable Transfection

Name:
Stable Transfection
Detailed information:
A form of transfection experiment designed to produce permanent lines of cultured cells with a new gene inserted into their genome. Usually this is done by linking the desired gene with a “selectable” gene, i.e. a gene which confers resistance to a toxin. Upon putting the toxin into the culture medium, only those cells which incorporate the resistance gene will survive, and essentially all of those will also have incorporated the experimenter's gene.