Sub-Cloning

Name:
Sub-Cloning
Detailed information:
If you have a cloned piece of DNA (say, inserted into a plasmid) and you need unlimited copies of only a part of it, you might “sub-clone” it. This involves starting with several million copies of the original plasmid, cutting with restriction enzymes, and purifying the desired fragment out of the mixture. That fragment can then be inserted into a new plasmid for replication. It has now been subcloned.