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Fluorescein diacetate

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Fluorescein diacetate

EINECS 209-877-6
CAS No. 596-09-8 Density 1.47 g/cm3
Solubility Melting Point 200-203 °C(lit.)
Formula C24H16O7 Boiling Point 604.7 °C at 760 mmHg
Molecular Weight 416.38 Flash Point 264 °C
Transport Information Appearance Light yellow powder
Safety 24/25-36/37/39-27-26 Risk Codes 36/37/38
Molecular Structure Molecular Structure of 596-09-8 (Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,3',6'-bis(acetyloxy)-) Hazard Symbols IrritantXi
Synonyms

Fluorescein,diacetate (6CI,7CI,8CI);3',6'-Diacetylfluorescein;F 1303;FDA;Fluorescein 3',6'-diacetate;MFCD 5062;NSC 4726;NSC 667259;

 

Fluorescein diacetate Specification

The Fluorescein diacetate ,its cas register number is 596-09-8.It also can be called as 3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl diacetate and the IUPAC name about this chemicals is (6'-Acetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate .It belongs to the following product categories, such as Colours, Dyes, Indicators & Pigments and so on.It can be stored under 20°C.

Following are the chemical properties about Fluorescein diacetate :(1)#H bond acceptors: 7 ; (2)#H bond donors: 0 ; (3)#Freely Rotating Bonds: 4 ; (4)Polar Surface Area: 88.13Å2 ; (5)Index of Refraction: 1.681 ; (6)Molar Refractivity: 107.16 cm3 ; (7)Molar Volume: 283.1 cm3 ; (8)Polarizability: 42.48x10-24cm3 ; (9)Surface Tension: 69.2 dyne/cm; (10)Enthalpy of Vaporization: 89.92 kJ/mol ; (11)Vapour Pressure: 1.42E-14 mmHg at 25°C

It is a dangerous chemical, because it irritating to eyes, respiratory system and skin. In the using process, you need to wear suitable protective clothing, gloves and eye/face protection and avoid contact with skin and eyes, also take off immediately all contaminated clothing, In addition, in case of contact with eyes, rinse immediately with plenty of water and seek medical advice. WGK Germany about this chemical is 3.

The Fluorescein diacetate is a light yellow powder, it also known as FDA, it can be used as fluorescein through the cell membrane to accumulate in living cells, As the hydrophilic of fluorescent is lower than BCECF or calcein, fluorescein leakage from the cell volume was also high.FDA is also used for flow cytometry. Fluorescence excitation and emission wavelengths were 488nm and 530nm.

This chemicals can be described computed from structure:
(1)Canonical SMILES: CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC=CC=C5C(=O)O3
(2)InChI: InChI=1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
(3)InChIKey: CHADEQDQBURGHL-UHFFFAOYSA-N

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