10.1002/anie.201802962
Angewandte Chemie International Edition
COMMUNICATION
Table 3: Biocatalytic conversion of substrate 8.
ee [%][b]
(R,R)-10
ee [%][b]
(S,R)-10
Entry
Enzyme
Conv. [%][a]
Selectivity [red:c]
de trans/cis [%]
1
2
3
4
5
OPR3 WT
OPR3 Y190F
OPR3 Y190W
YqjM WT
>99
>99
94
52:48
25:75
29:71
28:72
5:95
72
71
71
-30
94
>99
>99
>99
>99
>99
54
-43
-51
-8
81
YqjM Y169F
89
-29
[a] Conversions were determined by GC-FID analysis of the crude reaction mixture by using 1,2-DME as an internal standard. [b] For
assignment of ee products were analyzed as the corresponding alcohols after reduction of the samples using NaBH4.
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