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ChemComm
Page 4 of 4
DOI: 10.1039/C8CC04449J
COMMUNICATION
Journal Name
that deazaflavin dependent enzymes can be exploited for Zhang, S. Kang,; T. H. Keller, J. Jiricek, C. E. Barry, Science. 2008,
enantioselective ene reductions. The recently established 322, 1392.
expression of F420-dependent glucose-6-phosphate dehydro-
genase allowed the use of glucose-6-phophate for regene- L. A. J. Mur, Sci. Rep., 2018, 5084, 1-7.
rating reduced F420. The identification of various FDRs and an (13) C. Greening, T. Jirapanjawat, S. Afroze, B. Ney, C. Scott,
efficient F420 recycling system will facilitate future studies to G. Pandey, B. M. Lee, R. J. Russel, C. J. Jackson, J. G. Oakeshott,
explore this newly identified family of ene-reductases. One M. C. Taylor, C. W. Warden, Front Microbiol. 2017, , 1000.
challenge will be the discovery of new FDRs with a higher (14) E. H. Mashalidis A. G. Gittis A. Tomczak, C. Abell, C. E.
efficiency, because the catalytic rate observed for FDRs is Barry, D. N. Garboczi, Protein Sci. 2015, 24, 729-740.
rather low. Alternatively, it might be possible to fine-tune FDRs (15) G. V. Lapalikar, M. C. Taylor, A. C. Warden, C. Scott, R. J.
to enhance their biocatalytic activities through enzyme Russell, J. G. Oakeshott, PLoS One, 2012, , e30114.
engineering approaches. Once the crystal structures of FDRs (16) F. H. Ahmed, P. D. Carr, B. M. Lee, L. A. Jurnou, A. E.
(12) R. Baptista, D. M. Fazakerley, M. Beckmann, L. Baillie &
H
2
8
2
are solved, it would be interesting to compare the active site Mohamed, N-S Hong, J. Flanagan, M. C. Taylor, C. Greening, C.
of FDRs with OYE ene-reductases which may give leads to alter J. Jackson, . Mol. Biol. 2015, 22, 3554.
the enantioselectivity of FDRs by protein engineering. Another (17) S. E. Cellitti, J. Shaffer, D. H. Jones, T. Mukherjee, M.
challenge in this field will be the development of an efficient Gurumurthy, B. Bursulaya, H. I. Boshoff, I. Choi, A. Nayar, Y. S.
production system for the unusual F420 cofactor. Future Lee, Structure. 2012, 20, 101.
research will reveal whether these FDRs can be developed in (18) M. Gurumurthy, T. Mukherjee, C. S Dowd, R. Singh, P.
such a way that they can compete with or outperform the Niyomrattanakit, J. A. Tay, A. Nayyar, Y. S. Lee, J. Cherian, H. I.
currently used ene-reductases
Boshoff, T. Dick, C. E. Barry, U. H. Manjunatha, FEBS J. 2012,
2
79, 113.
(19) Q-T. Nguyen, G. Trinco, C. Binda, A. Mattevi, M. W.
Conflicts of interest
There are no conflicts to declare.
Fraaije, Appl. Microbiol. Biotechnol. 2017, 101, 2831.
20) T. Knaus, F. G. Mutti, L. D. Humphreys, N. J. Turnerb, N.
S. Scrutton Org. Biomol. Chem. 2015, 13, 223.
21) A. Scholtissek, D. Tischler, A. H. Westphal, W. J. H. van
Berkel, C. E. Paul, Catalysts 2017, , 130.
(
(
Acknowledgment
7
Funding from the Dutch Science Foundation (NWO-VICI project
M.W. Fraaije) is gratefully acknowledged.
References
(
1) H. S. Toogood, N. S. Scrutton, Curr. Opin. Chem. Biol.,
014, 19, 107.
2) H. S. Toogood, J. S. Gardiner, N. S. Scrutton,
ChemCatChem., 2010, , 892.
3) C. K. Winkler, K. Faber, M. Hall. Curr. Opin. Chem. Biol.,
018, 43, 97.
4) C. T. Walsh, T. A. Wencewicz, Nat. Prod. Rep., 2013, 30
75.
5) C. Greening, F. H. Ahmed, A. E. Mohamed, B. M. Lee, G.
2
(
2
(
2
1
(
,
(
Pandey, A. C. Warden, C. Scott, J. G. Oakeshott, M. C. Taylor, C.
Jackson, Microbiol. Mol. Biol. Rev., 2016, 80, 451.
(6) B. Ney, F. H. Ahmed, C. R. Carere, A. Biswas, A. C.
Warden, S. E. Morales, G. Pandey, S. J. Watt, J. G. Oakeshott,
M. C. Taylor, M. B. Stott, C. J. Jackson, C. Greening, ISME J.,
2
017, 11, 125.
7) J. Novotnfi, J. Neuzil, Z. Hostalek, FEMS Microbiol. Lett.
989, 59, 241.
8) P. Wang, G. Bashiri, X. Gao, M. R. Sawaya,; Y. J. Tang, Am.
Chem. Soc., 2013, 135, 7138.
9) M. C. Taylor, C. J. Jackson, D. B. Tattersall, N. French, T. S.
Peat, J. Newman, L. J. Briggs, G. V. Lapalikar, P. M.Campbell, C.
Scott, R. J. Russel, J. G. Oakeshott, Mol. Microbiol. 2010, 78
61.
10) M. Gurumurthy, M. Rao, T. Mukherjee, S. P. S. Rao, H.
(
1
(
(
,
5
(
I.Boshoff, T. Dick, C. E. Barry, U. H. Manjunatha, Mol.
Microbiol. 2013, 87, 744.
(11) R. Singh, U. Manjunatha, H. I. Boshoff, Y. H. Ha, P.
Niyomrattanakit, R. Ledwidge, C. S. Dowd, I. Y. Lee, P. Kim, L.
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