10.1002/chem.201805436
Chemistry - A European Journal
FULL PAPER
See supporting information file.
Acknowledgements
The authors gratefully acknowledge Università Ca‘ Foscari for
support.
Keywords: bisphosphonate • diketopyrrolopyrrole • fluorescent
probe • osteoporosis • in vitro imaging
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Figure 5: Fluorescence microscopy of FLUOProbe treated bovine cortical bone.
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cells (cultured with α-MEM by Life Technologies) supplemented
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Figure 6: Osteoclast resorbing activity tests. Untreated (A) and FLUOProbe-
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Conclusions
In this contribution we reported about the synthesis of a conjugate
between an unusual red fluorescent diketopyrrolopyrrole (DPP)
unit and a bisphosphonate (BP) precursor using the approach to
provide a new tool to target bone and monitor the interaction. After
deep investigation, conjugation through a triazole unit between -
azido rather than -azido BP and an alkyne functionalized DPP
fluorophore group was found to be successful. Visualization of the
DPP-BP conjugate on osteoclasts as well as the specific anti-
resorption activity was successfully demonstrated.
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Experimental Section
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