Angewandte Chemie International Edition
10.1002/anie.201804802
COMMUNICATION
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Figure 4. The selectivity of cancer cell capture on the dynamic biointerface. a)
The representative fluorescent images of a mixed cell suspension containing
1:1 MCF-7 cells (green, DiO) and HL60 cells (red, Dil). b) The surface with
(
DOPA) -S -WxEAAYQrFLcould selectively capture MCF-7 cells from the
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mixed cell suspension. Scale bar is 100 μm.
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surface and almost no HL60 cells could be captured (Figure 4).
Quantitative studies revealed that the proportion of MCF-7 cells
among the captured cells was as high as 98.1 ± 0.7%. This result
confirmed the ability of our dynamic biointerface to selectively
recognize and capture targeted cancer cells. Moreover, we can
imagine that the flexibility of using mussel-inspired peptides with
different cell-targeting sequences will endow the dynamic
biointerface with desired cell selectivity.
[
[
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In summary, we have developed a mussel-inspired dynamic
biointerface based on reversible catechol-boronate chemistry.
Dynamic presentation of cell-binding motifs at the interface can
be easily achieved through sugar-responsive catechol-boronate
interactions between biomimetic peptides and PBA polymer-
grafted substrates. Moreover, by rational design of mussel-
inspired peptides capped with different cell-binding sequences,
the biointerface enables not only dynamic modulation of stem cell
adhesion behaviors, but also selective isolation of tumor cells.
Therefore, our mussel-inspired dynamic biointerface holds great
promise in areas ranging from fundamental cell biology research
to cell-based medical applications.
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Acknowledgements
We acknowledge the financial support from the National Natural
Science Foundation of China (21574091, 21573097 and
51503087), and the Natural Science Foundation of Jiangsu
Province (BK20160056).
Keywords: dynamic biointerface • mussel-inspired peptides •
cell adhesion • tumor cells • cell capture and release
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