Acrylamide-Coumarin-Benzaldehyde as a Turn-On Fluorescent Probe for
Detection of Cys/Hcy
BULLETIN OF THE
Article
KOREAN CHEMICAL SOCIETY
addition reaction of acrylamide group of 1 with Cys/Hcy.
The detection mechanism of 1 for Cys/Hcy was identified
by ESI-MS and H NMR analyses. Additionally, the fluo-
rescence enhancement of 1 was selectively monitored to
Cys/Hcy over other biologically potential interferants
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Besides, 1 can give fluorescence turn-on for Cys/Hcy in the
wide pH range of 2-11 and the limit of detections (LOD)
for Cys and Hcy turned out to be 17.25 and 8.69 μM. As a
result, we could suggest that fluorescent probe 1 would be
widely applied to monitoring Cys and Hcy present in most
physiological conditions.
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Acknowledgments. This work was supported by the Med-
ical Research Center Program (No. 2011-0030074) through
National Research Foundation (NRF) grant funded by the
Korean government (MSIP).
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Bull. Korean Chem. Soc. 2019
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