water; 150 lL) was added to the wells and the plate was shaken
at room temperature for 15 min, the wells were washed with
water (3 × 150 lL), SDS (10% in water; 150 lL) was added
and the plate was again shaken at room temperature for 15 min.
The wells were washed with water (3 × 150 lL) and HRP-AV
28 S. Park and I. Shin, Angew. Chem., Int. Ed., 2002, 41, 3180–
3182.
2
3
9 S. Park, M. R. Lee, S. J. Pyo and I. Shin, J. Am. Chem. Soc., 2004,
1
26, 4812–4819.
0 E. A. Yates, M. O. Jones, C. E. Clarke, A. K. Powell, S. R. Johnson,
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−
1
(
4 lg mL ; 0.05% Tween 20 in PBS; 100 lL) was added. The
1
3, 2061–2063.
plate was shaken at room temperature for 35 min and substrate
solution was added and the absorbance measured as before. The
31 E. W. Adams, J. Ueberfeld, D. M. Ratner, B. R. O’Keefe, D. R.
Walt and P. H. Seeberger, Angew. Chem., Int. Ed., 2003, 42, 5317–
5
320.
wells were washed with water (3 × 150 lL), NaHCO
3
(sat. aq;
3
2 M. C. Bryan, F. Fazio, H.-K. Lee, C.-Y. Huang, A. Chang, M. D.
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2
× 150 lL), SDS (1% in water; 3 × 150 lL) and water again
(
4 × 150 lL). SDS (10% in water; 150 lL) was added to the
wells and the plate was shaken at room temperature for 15 min,
the wells were washed with water (3 × 150 lL), more SDS (10%
in water; 150 lL) was added and the plate was again shaken
at room temperature for 15 min. The wells were washed with
water (3 × 150 lL), blocked with BSA and RCA120, HRP-AV
was added and the absorbance was measured as before.
3
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