Chemical Property of 2-(3,6-Diacetoxy-2,7-dichloro-9h-xanthen-9-yl)benzoic acid
Chemical Property:
- Melting Point:209-210 °C(lit.)
- Boiling Point:593.5 °C at 760 mmHg
- PKA:3.79±0.36(Predicted)
- Flash Point:312.7 °C
- PSA：99.13000
- Density:1.463g/cm3
- LogP:5.82810
- Storage Temp.:−20°C
- Solubility.:DMF: soluble
- XLogP3:5.2
- Hydrogen Bond Donor Count:1
- Hydrogen Bond Acceptor Count:7
- Rotatable Bond Count:6
- Exact Mass:486.0273082
- Heavy Atom Count:33
- Complexity:717
- Purity/Quality:
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99%, *data from raw suppliers
2′,7′-Dichlorofluorescin diacetate ≥97% *data from reagent suppliers
Safty Information:
- Pictogram(s):
Xi
- Hazard Codes:Xi
- Statements:
36/37/38
- Safety Statements:
22-24/25-26
- MSDS Files:
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Total 1 MSDS from other Authors
Useful:
- Canonical SMILES:CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl
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Description
DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M?1cm?1).
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Uses
Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide. 2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation. 2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
