Chemical Property of sodium 4-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate
Chemical Property:
- Appearance/Colour:pale powder
- PSA：266.62000
- LogP:4.05220
- Storage Temp.:under inert gas (nitrogen or Argon) at 2-8°C
- Hydrogen Bond Donor Count:0
- Hydrogen Bond Acceptor Count:13
- Rotatable Bond Count:4
- Exact Mass:599.99814188
- Heavy Atom Count:40
- Complexity:1080
- Purity/Quality:
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99% *data from raw suppliers
WST-8 *data from reagent suppliers
Safty Information:
- Pictogram(s):
- Hazard Codes:
- MSDS Files:
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SDS file from LookChem
Useful:
- Canonical SMILES:COC1=C(C=CC(=C1)[N+](=O)[O-])[N+]2=NC(=NN2C3=CC=C(C=C3)[N+](=O)[O-])C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+]
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description
WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt), a highly stable and water-soluble WST, is utilized incell Counting Kit-8 (CCK-8).It is more sensitive than WST-1 particularly at neutralpH. Because of the electron mediator, 1-methoxy PMSin this kit is highly stable, and CCK-8 is stable for at least six months at the room temperature and for one year at 0-5°C (asshown in Figure 1).Advantages: WST-8 is not cell permeable, which results in low cytotoxicity. Therefore,after the assay, it is possible to continue further experiments using the same cells.Furthermore, it produces the water-soluble formazan upon cellular reduction，which would provide an additional advantage to the method by allowing a simpler assay procedure and not required an extra step to dissolve the formazan.Disadvantages: An important consideration is that reduction of assay substrates isimpacted by changes in intracellular metabolic activity that has no direct effect on over-all cell viability.
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Uses
A highly sensitive tetrazolium reagent that produces a water-soluble formazan (orange color that absorbs at 460 nm) in NADH. It is newer generation formazan-based dyes which release the converted prod
uct into the medium in a soluble form allowing for a non-destructive determination of viability enabling the cells to be subject to further investigations. WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH.
