DIHYDROETHIDIUM

Base Information

• Chemical Name: DIHYDROETHIDIUM
• CAS No.: 104821-25-2
• Molecular Formula: C21H21N3
• Molecular Weight: 315.418
• Hs Code.: 29339900
• DSSTox Substance ID: DTXSID90909235
• Nikkaji Number: J69.852K
• Wikidata: Q3027884
• Mol file: 104821-25-2.mol

Synonyms:3,8-Phenanthridinediamine,5-ethyl-5,6-dihydro-6-phenyl-, (?à)-;Dihydroethidium;Hydroethidine;PD-MY 003;

Chemical Property of DIHYDROETHIDIUM

Chemical Property:

• Appearance/Colour: pink to purple fine crystalline powder
• Vapor Pressure: 1.83E-13mmHg at 25°C
• Refractive Index: 1.68
• Boiling Point: 580.4 °C at 760 mmHg
• PKA: 5.30±0.40(Predicted)
• Flash Point: 299.5 °C
• PSA: 55.92000
• Density: 1.197 g/cm³
• LogP: 5.29420
• Storage Temp.: −20°C
• Solubility.: acetonitrile: soluble
• XLogP3: 3.9
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 2
• Exact Mass: 315.173547683
• Heavy Atom Count: 24
• Complexity: 419

Purity/Quality:

99% ^*data from raw suppliers

Dihydroethidium ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: CCN1C(C2=C(C=CC(=C2)N)C3=C1C=C(C=C3)N)C4=CC=CC=C4
• Uses: DIHYDROETHIDIUM is redox indicator. Blue fluorescence until oxidized to ethidium. Can be used for detecting superoxide radical in cells, tissues and organisms. Dihydroethidium, also known as hydroethidine, is a dye that can permeate viable cells and accumulate in the nucleus after it is dehydrogenated to ethidium bromide and is used to measure oxygen reduction in phagocytes during respiratory burst. In the presence of an oxidant it will fluoresce and has been used as a dye in flow cytometry. Studies have used Dihydroethidium to study alveolar macrophages, blood leukocytes, and epithelial cells, among others, and has be used to measure intracellular H2O2 and O2-, and reactive oxygen. Dyes and metabolites.

Technology Process of DIHYDROETHIDIUM

There total 1 articles about DIHYDROETHIDIUM which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

ethidium Bromide (1239-45-8) → dihydroethidium (104821-25-2,38483-26-0)

Guidance literature:

With sodium tetrahydroborate; In methanol; at 0 ℃; for 24h;

DOI:10.1016/S0040-4020(01)81017-6

Reference yield:

dihydroethidium (104821-25-2,38483-26-0) → ethidium (3546-21-2,89160-73-6)

Guidance literature:

With superoxide dismutase; xanthine oxidase; xanthin; Reagent/catalyst; Kinetics; Enzymatic reaction;

DOI:10.1002/anie.201002228

Reference yield:

dihydroethidium (104821-25-2,38483-26-0) → 2-hydroxy-ethidium cation (854670-32-9) + ethidium (3546-21-2,89160-73-6)

Guidance literature:

With glucose dehydrogenase; 2-hydroxynaphtho-1,4-quinone; D-glucose; 5-hydroxynaphtho-1,4-quinone; His₁₀-tagged NfsB; oxygen; NADPH; In aq. buffer; at 20 ℃; for 24h; pH=7.8; Kinetics; Darkness;

DOI:10.1002/cbic.201800071

upstream raw materials:

ethidium Bromide

Downstream raw materials:

N-3,8-di(BOC glycyl)amino-6-ethyl-5-phenyl-5,6-dihydrophenantridine

N-[5-Ethyl-3-(3-methoxycarbonyl-propionylamino)-6-phenyl-5,6-dihydro-phenanthridin-8-yl]-succinamic acid methyl ester

5-Ethyl-3,8-bis-(3-methoxycarbonyl-propionylamino)-6-phenyl-phenanthridinium; iodide

N-3,8-di(BOC glycyl) ethidium iodide

Refernces

• 1、 Loccufier,Schacht. "Regioselective acylation of 3,8-diamino-5-ethyl-6-phenyl-phenantridium bromide preparation of potential new trypanocides". . p. 3385 - 3396. Retrieved 2007/10/02.