Synonyms:NSC 607770;NSC 607771;NSC 607772;
Osmium *data from reagent suppliers
The research focuses on the synthesis and characterization of new dithioformato complexes of platinum metals, specifically ruthenium, osmium, and iridium. The purpose of the study was to explore the "insertion" of carbon disulfide into platinum metal-hydrogen bonds, leading to the formation of a range of dithioformato complexes. The researchers used various chemical species, including [MX(S2CH)(CO)(PPh3)2] (with M being Ru or Os and X being Cl, Br, or OCOCF3), [M(S2CH)2(PPh3)2], [IrCl2(S2CH)(PPh3)2], and others, to investigate the stereochemistry and structure of these complexes. The conclusions drawn from the study were that the dithioformate anion could be detected and characterized by its IR and proton NMR spectra, and that the stereochemistry of the complexes could be assigned based on NMR patterns and couplings. The research also established that the dithioformate ligands exhibit specific NMR couplings that are valuable for determining the stereochemistry of the complexes. The study provided a comprehensive series of dithioformato complexes, contributing to the understanding of their synthesis, structure, and potential applications.
The research aims to develop a facile synthesis method for hydroxymethylcytosine (hmC)-containing oligonucleotides (ODNs) and investigate their reactivity upon osmium oxidation. The study synthesizes hmC-containing ODNs using a straightforward route starting from thymidine and involving protection, bromination, and amination steps, ultimately converting the nucleoside into phosphoramidite form for DNA autosynthesizer use. The synthesized ODNs form stable duplexes with complementary DNA, exhibiting similar melting temperatures and enzymatic digestion properties to methylated counterparts. Osmium oxidation, a method previously used for detecting 5-methylcytosine (mC), is tested on hmC-containing ODNs under specific reaction conditions, revealing that hmC is oxidized as efficiently as mC, forming a stable ternary complex. The study concludes that osmium oxidation is a viable method for detecting hmC in DNA, potentially advancing epigenetic studies. Key chemicals used include thymidine, acetic anhydride, N-bromosuccinimide, 3-hydroxypropionitrile, phosphorus oxychloride, ammonia, di(n-butyl)formamidine, potassium osmate, potassium hexacyanoferrate(III), and bipyridine.
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