Propidium iodide

Base Information

• Chemical Name: Propidium iodide
• CAS No.: 25535-16-4
• Molecular Formula: C27H34 N4 . 2 I
• Molecular Weight: 668.403
• Hs Code.: 29339900
• European Community (EC) Number: 247-081-0
• UNII: TP416O228T
• DSSTox Substance ID: DTXSID20894084
• Wikipedia: Propidium_iodide
• NCI Thesaurus Code: C72903
• ChEMBL ID: CHEMBL345124
• Mol file: 25535-16-4.mol

Synonyms:Diiodide, Propidium;Iodide, Propidium;Propidium;Propidium Diiodide;Propidium Iodide

Chemical Property of Propidium iodide

Chemical Property:

• Melting Point: 220-225oC
• Boiling Point: °Cat760mmHg
• Flash Point: °C
• PSA: 55.92000
• Density: g/cm³
• LogP: 0.15880
• Storage Temp.: 2-8°C
• Sensitive.: Light Sensitive & Hygroscopic
• Solubility.: Difficult to mix.
• Water Solubility.: Soluble in water at 1mg/ml
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 7
• Exact Mass: 668.08729
• Heavy Atom Count: 33
• Complexity: 555

Purity/Quality:

98%,99%, ^*data from raw suppliers

Propidium Iodide ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xi,Xn
• Hazard Codes: Xi,Xn
• Statements: 36/37/38-68-67-48/20/22-40-36/38-22-20-63
• Safety Statements: 26-36-22-36/37

MSDS Files:

Total 1 MSDS from other Authors

Useful:

• Chemical Classes: Other Uses -> Biochemical Research
• Canonical SMILES: CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]
• Description: Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.
• Uses: 1 mg/mL in water Propidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes. Antibacterial;Detergent Propidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process. Used as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability

Technology Process of Propidium iodide

There total 1 articles about Propidium iodide which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

→ propidium iodide (25535-16-4)

Guidance literature:

With sulfuric acid; anschliessend mit wss. NH₃ und KI;

Reference yield: 92.0%

propidium iodide (25535-16-4) + lithium bis((pentafluoroethyl)sulfonyl)amide → C27H34N4(2+)*2C4F10NO4S2(1-)

Guidance literature:

In dichloromethane; water; at 20 ℃; for 6h;

DOI:10.1021/acs.jpca.8b06948

Reference yield:

propidium iodide (25535-16-4) → hydropropidine (1418010-07-7)

Guidance literature:

With sodium tetrahydroborate; In methanol; at 0 ℃;

DOI:10.1016/j.freeradbiomed.2012.09.018

Downstream raw materials:

propidium

dipropidium

C₅₄H₆₈N₈⁽²⁺⁾

C₅₄H₆₇N₈⁽³⁺⁾