HEMOCYANIN, KEYHOLE LIMPET

Base Information

• Chemical Name: HEMOCYANIN, KEYHOLE LIMPET
• CAS No.: 9013-72-3
• Molecular Weight: 0

Synonyms:Hemocyanin;Cyanins;

Chemical Property of HEMOCYANIN, KEYHOLE LIMPET

Chemical Property:

• PSA: 0.00000
• LogP: 0.00000
• Storage Temp.: 2-8°C
• Solubility.: H2O: soluble

Purity/Quality:

98%,99%, ^*data from raw suppliers

Keyhole Limpet Hemocyanin ^*data from reagent suppliers

Safty Information:

• Statements: 52/53
• Safety Statements: 23-24/25-61

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Uses: Hemocyanin from Limulus polyphemus hemolymph has been used:in electrophoretic field gradient focusing as a reference standard for optimizing fluorescence quenching for noncolored proteinsas standard protein for pH based crystallization screening experimentsin peptide conjugation studies Keyhole limpet hemocyanin (KLH) is a strong and well known immune-stimulant in both experimental animals and humans. It has been used as a hapten carrier protein to enhance antigen-specific T cell priming and is known to stimulate a CD4+ T-cell response.Hemocyanin was used to study if antipeptide antibodies specifically recognize the l1 protein of the human papilloma virus of type 31. It was also used to study the acid-induced unfolding of didecameric keyhole limpet hemocyanin.

Refernces

Development of an enzyme-linked immunosorbent assay for the detection of the organophosphorus insecticide acephate

10.1021/jf021020i

The study developed a competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the detection of the organophosphorus insecticide acephate. Five different haptens mimicking acephate were synthesized and conjugated with carrier proteins to raise polyclonal antibodies in rabbits. These antibodies were then used to detect acephate in various samples. The study evaluated the effects of different assay conditions on sensitivity and found an IC50 value of 25 ng/mL for acephate, with a detection range of 5-140 ng/mL and a lowest detection limit of 2 ng/mL. The assay showed low cross-reactivities with structurally related insecticides, indicating high specificity. The chemicals used in the study included acephate, various haptens, bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), and organic solvents for assay optimization. These chemicals served to mimic the target analyte, carry the haptens in the assay, and adjust assay conditions to optimize sensitivity and specificity for detecting acephate residues in environmental and agricultural samples.

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