CY3 NHS ESTER

Base Information

• Chemical Name: CY3 NHS ESTER
• CAS No.: 146368-16-3
• Molecular Formula: C35H41 N3 O10 S2
• Molecular Weight: 727.84400
• Hs Code.: 29349990

Synonyms:3H-Indolium,2-[3-[1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1-propenyl]-1-ethyl-3,3-dimethyl-5-sulfo-,inner salt (9CI); Cy3; Cy3 NHS Ester; FluoroLink Mono Reactive Dye Cy3

Chemical Property of CY3 NHS ESTER

Chemical Property:

• PSA: 198.26000
• LogP: 6.76230
• Storage Temp.: under inert gas (nitrogen or Argon) at 2-8°C

Purity/Quality:

99%, ^*data from raw suppliers

Cy3NHSEster ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Uses: Fluorescent dyes are typically used for imaging applications. Cy3 NHS Ester is a fluorescent dye suitable for labeling proteins and nucleic acids. It exhibits excitation and emission at 555 nm and 570 nm, respectively. Cy3 NHS Ester can be used to label functional nucleic acids to detect tertiary structure folding such as guanine quadruplex.

Technology Process of CY3 NHS ESTER

There total 2 articles about CY3 NHS ESTER which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

C31H38N2O8S2 (1240125-28-3,146368-13-0,235749-10-7,666847-58-1) + O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate (105832-38-0) → Cy3 N-hydroxysuccinimidyl ester (1240125-45-4,146368-16-3)

Guidance literature:

With N-ethyl-N,N-diisopropylamine; In 1-methyl-pyrrolidin-2-one; at 20 ℃; for 1h; Darkness;

DOI:10.1039/c0ob00133c

Reference yield:

3H-indolium-1-(5-carboxypentyl)-2-[(1E,3E)-3-(1-ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl-5-sulfo-, inner salt (235749-10-7,146368-13-0,666847-58-1,1240125-28-3) + O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate (105832-38-0) → 3H-indolium-1-(5-carboxypentyl)-2-[(1E,3E)-3-(1-ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl-5-sulfo-, inner salt, succinimidyl ester (146368-16-3)

Guidance literature:

With 1-methyl-pyrrolidin-2-one; N-ethyl-N,N-diisopropylamine; at 20 ℃; for 1h;

DOI:10.1002/chem.200501538

Reference yield: 90.0%

3H-indolium-1-(5-carboxypentyl)-2-[(1E,3E)-3-(1-ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl-5-sulfo-, inner salt, succinimidyl ester (146368-16-3) + Fmoc-gabapentin-choline (1152425-52-9) → Cy3-gabapentin-choline (1152425-53-0)

Guidance literature:

With dmap; In N,N-dimethyl-formamide; at 20 ℃;

DOI:10.1016/j.tetlet.2009.02.125

upstream raw materials:

3H-indolium-1-(5-carboxypentyl)-2-[(1E,3E)-3-(1-ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl-5-sulfo-, inner salt

O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate

C₃₁H₃₈N₂O₈S₂

Downstream raw materials:

Cy₃-gabapentin-choline

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