Cholesterol oxidase

Base Information

• Chemical Name: Cholesterol oxidase
• CAS No.: 9028-76-6
• Molecular Formula: NULL
• Molecular Weight: 0
• Hs Code.: 35079090
• Mol file: 9028-76-6.mol

Synonyms:3-Hydroxysteroidoxidase;Cholesterin oxidase;3beta-Hydroxysteroid:oxygen oxidoreductase;E.C. 1.1.3.6;3beta-Hydroxy steroid oxidoreductase;Cholesterol- O2 oxidoreductase;Cholesterol:oxygen oxidoreductase;

Chemical Property of Cholesterol oxidase

Chemical Property:

• Appearance/Colour: pale yellow solution (slightly hazy)
• PSA: 0.00000
• LogP: 0.00000
• Storage Temp.: 2-8°C
• Solubility.: 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

Purity/Quality:

99%, ^*data from raw suppliers

Cholesterol Oxidase, Cholesterol Oxidase, ^*data from reagent suppliers

Safty Information:

• Safety Statements: 24/25

MSDS Files:

SDS file from LookChem

Useful:

• Uses: Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease. Cholesterol oxidases are part of a unique class of enzymes that are soluble proteins although they interact with highly insoluble substrates. The natural substrate, cholesterol, is an important membrane component exhibiting low solubility in the aqueous medium of the cell, hence the enzyme must interact with the lipid bilayer in order to for the substrate to partition out of the membrane and undergo oxidation at the enzyme active site. In this regard cholesterol oxidase is an interfacial enzyme as it binds transiently to the membrane surface during catalysis and can only access the substrate from the membrane phase. Other examples of interfacial enzymes include phospholipases (Berg et al., 2001; Gelb et al., 1995; Jain et al., 1995). Cholesterol oxidase has a number of important commercial applications. Initial studies on the enzyme focused on its use for the determination of cholesterol in serum, HDL or LDL (see review, Smith and Brooks, 1976). Serum cholesterol levels are determined using a three-enzyme assay, including cholesterol esterase, cholesterol oxidase and peroxidase (Richmond, 1973; Allain et al., 1974; Richmond, 1976). More recently electrochemical biosensors with immobilized cholesterol oxidase have been employed to determine cholesterol levels in serum or food (Arya et al., 2007; Basu et al., 2007; Vidal et al., 2004). Determining the serum cholesterol levels is critical for the assessment of a variety of diseases including hypercholesterolemia, coronary heart disease and lipid disorders for estimating the risk of thrombosis and myocardial infarction. Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.