MUNG BEAN NUCLEASE

Base Information

• Chemical Name: MUNG BEAN NUCLEASE
• CAS No.: 9025-65-4
• Molecular Weight: 0
• Mol file: 9025-65-4.mol

Synonyms:AzotobacterNuclease; E.C. 3.1.30.2; E.C. 3.1.4.9; EC 3.1.30.2; Genease I; Mung beannuclease; Nuclease of Mung bean; Nucleate endonuclease; Serratia endonuclease;Serratia marcescens extracellular nuclease; Serratia marcescens nuclease

Chemical Property of MUNG BEAN NUCLEASE

Chemical Property:

• PSA: 0.00000
• LogP: 0.00000
• Storage Temp.: 2-8°C

Purity/Quality:

98%,99%, ^*data from raw suppliers

Turbonuclease from Serratia marcescens recombinant, expressed in E. coli ^*data from reagent suppliers

Safty Information:

• Safety Statements: 23-24/25

MSDS Files:

Total 1 MSDS from other Authors

Useful:

• Uses: The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates. The enzyme from Sigma has been used to limit cell clumping during the preparation of chimeric cell mixtures. It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA. Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.