BIOTIN-MALEIMIDE

Base Information

• Chemical Name: BIOTIN-MALEIMIDE
• CAS No.: 116919-18-7
• Molecular Formula: C₂₀H₂₉N₅O₅S
• Molecular Weight: 451.547
• Hs Code.: 29349990
• Mol file: 116919-18-7.mol

Synonyms:1H-Thieno[3,4-d]imidazole-4-pentanoicacid, hexahydro-2-oxo-,2-[6-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)-1-oxohexyl]hydrazide, [3aS-(3aa,4b,6aa)]-; Biotin-maleimide

Chemical Property of BIOTIN-MALEIMIDE

Chemical Property:

• Vapor Pressure: 2.38E-27mmHg at 25°C
• Boiling Point: 825ºC at 760 mmHg
• PKA: 11.59±0.50(Predicted)
• Flash Point: 452.8 ºC
• Density: 1.286 g/cm³
• Storage Temp.: 2-8°C
• Solubility.: acetic acid: 20 mg/mL

Purity/Quality:

98%,99%, ^*data from raw suppliers

Biotin-maleimide ≥95% (TLC), powder ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

Useful:

• Uses: Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides.

Technology Process of BIOTIN-MALEIMIDE

There total 3 articles about BIOTIN-MALEIMIDE which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

N-(ε-maleimidocaproic acid) hydrazide (81186-33-6) + biotin chloride (91853-90-6) → N-biotinoyl-N′-(6-maleimidohexanoyl)hydrazide (116919-18-7)

Guidance literature:

With triethylamine; In tetrahydrofuran; at 20 ℃; for 8h;

Reference yield:

2,5-Dihydro-2,5-dioxo-1H-pyrrole-1-hexanoic acid (55750-53-3) → N-biotinoyl-N′-(6-maleimidohexanoyl)hydrazide (116919-18-7)

Guidance literature:

Multi-step reaction with 3 steps

1: dicyclohexyl-carbodiimide / dichloromethane / 4 h / 20 °C

2: hydrazine hydrate / tetrahydrofuran / 4 h / 20 °C

3: triethylamine / tetrahydrofuran / 8 h / 20 °C

With hydrazine hydrate; triethylamine; dicyclohexyl-carbodiimide; In tetrahydrofuran; dichloromethane;

Reference yield:

6-maleimidohexanoic acid N-hydroxylsuccinimide ester (55750-63-5) → N-biotinoyl-N′-(6-maleimidohexanoyl)hydrazide (116919-18-7)

Guidance literature:

Multi-step reaction with 2 steps

1: hydrazine hydrate / tetrahydrofuran / 4 h / 20 °C

2: triethylamine / tetrahydrofuran / 8 h / 20 °C

With hydrazine hydrate; triethylamine; In tetrahydrofuran;

upstream raw materials:

2,5-Dihydro-2,5-dioxo-1H-pyrrole-1-hexanoic acid

6-maleimidohexanoic acid N-hydroxylsuccinimide ester

N-(ε-maleimidocaproic acid) hydrazide

biotin chloride

Refernces