Alkaline phosphatase

Base Information

• Chemical Name: Alkaline phosphatase
• CAS No.: 9001-78-9
• Deprecated CAS: 119940-17-9,131754-85-3
• Molecular Formula: Unspecified
• Molecular Weight: 496.56054
• Hs Code.: 35079090
• European Community (EC) Number: 232-631-4
• Mol file: 9001-78-9.mol

Synonyms:Alkaline Phosphatase

Chemical Property of Alkaline phosphatase

Chemical Property:

• PSA: 0.00000
• Density: 1.12 g/mL at 20 °C
• LogP: 0.00000
• Storage Temp.: 2-8°C
• Water Solubility.: It is soluble in water.
• XLogP3: -5
• Hydrogen Bond Donor Count: 6
• Hydrogen Bond Acceptor Count: 8
• Rotatable Bond Count: 11
• Exact Mass: 496.27578090
• Heavy Atom Count: 35
• Complexity: 846

Purity/Quality:

98%min ^*data from raw suppliers

Phosphatase, Alkaline, Calf Intestine ^*data from reagent suppliers

Safty Information:

• Pictogram(s): B,Xi
• Hazard Codes: B,Xi
• Statements: 36/37/38
• Safety Statements: 26-36-24/25-22-23

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Canonical SMILES: CC(C(=O)N1CCCC1C(=O)NCC(=O)N2CCCC2C(=O)NC(CCCN=C(N)N)C(=O)O)N
• Recent ClinicalTrials: Bovine Intestinal Alkaline Phosphatase for the Treatment of Patients With Sepsis
• Recent EU Clinical Trials: Safety and preliminary efficacy of Alkaline Phosphatase in severely burned patients
• Uses: Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes. Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids. Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.

Refernces

Rapid and efficient syntheses of phosphorylated dinucleotides

10.1080/15257779408011881

The study presents a solution phase approach to synthesize phosphorylated dinucleotides, which are potentially useful therapeutic agents and important for studying weak interactions in molecular systems. The researchers developed nine or ten-step syntheses to produce the dimers dpApA, dApAp, dpTpT, and dTpTp in yields of 49%, 45%, 32%, and 20% respectively. The synthesis involves several key chemicals: nucleoside phosphines (4 and 5) react with protected nucleosides (6 or 7) to form fully protected dimers (8 and 9). These dimers are then selectively deprotected to yield dinucleotides with a deblocked 3'- or 5'-OH (10-13). A p-nitrophenethylphosphine derivative (14) is used to add a terminal phosphate group, leading to the formation of compounds (15-18). Further reactions, including oxidation and removal of protecting groups, result in the final phosphorylated dinucleotides (21, 22, 24, and 27). The synthetic intermediates were characterized by 1H and 31P NMR, and the structures of the final products were confirmed by hydrolysis with alkaline phosphatase and comparison with authentic samples.