Succinyl-alanyl-alanyl-prolyl-phenylalanine-p-nitroanilide

Base Information

• Chemical Name: Succinyl-alanyl-alanyl-prolyl-phenylalanine-p-nitroanilide
• CAS No.: 70967-97-4
• Molecular Formula: C30H36 N6 O9
• Molecular Weight: 624.651
• Hs Code.: 2933998090
• DSSTox Substance ID: DTXSID90991271
• Wikidata: Q82980978
• Mol file: 70967-97-4.mol

Synonyms:SAAPPNA;Suc-AAPF-pNA;Suc-Ala-Ala-Pro-Phe-4-NA;succinyl-alanyl-alanyl-prolyl-phenylalanine-4-nitroanilide;succinyl-alanyl-alanyl-prolyl-phenylalanine-p-nitroanilide

Chemical Property of Succinyl-alanyl-alanyl-prolyl-phenylalanine-p-nitroanilide

Chemical Property:

• Vapor Pressure: 0mmHg at 25°C
• Boiling Point: 1072.8oC at 760 mmHg
• Flash Point: 602.6oC
• PSA: 219.83000
• Density: 1.365g/cm3
• LogP: 2.83280
• Storage Temp.: −20°C
• Solubility.: DMF: 25 mg/mL, clear, light yellow
• XLogP3: 1.8
• Hydrogen Bond Donor Count: 5
• Hydrogen Bond Acceptor Count: 10
• Rotatable Bond Count: 13
• Exact Mass: 624.25437675
• Heavy Atom Count: 45
• Complexity: 1100

Purity/Quality:

98%,99%, ^*data from raw suppliers

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide ^*data from reagent suppliers

Safty Information:

• Safety Statements: 22-24/25

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Canonical SMILES: CC(C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(=O)C(CC2=CC=CC=C2)NC3=CC=C(C=C3)[N+](=O)[O-])NC(=O)CCC(=O)O
• Isomeric SMILES: C[C@@H](C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)NC(=O)[C@H](CC2=CC=CC=C2)NC3=CC=C(C=C3)[N+](=O)[O-])NC(=O)CCC(=O)O
• Description: Suc-AAPF-pNA is a chromogenic substrate that can be cleaved by cathepsin G (Km = 1.7 mM), subtilisins, chymotrypsin (Km = 60 μM), chymase (Km = 4 mM), and cyclophilin, but not neutrophil elastase. Release of p-nitroanilide is monitored at 405-410 nm. This substrate can be used for inhibitor screening and kinetic analysis.
• Uses: N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used as a substrate to determine chymotrypsin activity.

Technology Process of Succinyl-alanyl-alanyl-prolyl-phenylalanine-p-nitroanilide

There total 1 articles about Succinyl-alanyl-alanyl-prolyl-phenylalanine-p-nitroanilide which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

→ N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine p-nitroanilide (70967-97-4)

Guidance literature:

Reference yield:

N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine p-nitroanilide (70967-97-4) → 4-nitro-aniline (100-01-6,104810-17-5)

Guidance literature:

With Tris buffer; water; subtilisin; Kinetics; var. of enzyme, pH, further in D₂O, D₂O-H₂O;

DOI:10.1039/c39950001587

Reference yield:

N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine p-nitroanilide (70967-97-4) → 4-nitro-aniline (100-01-6,104810-17-5) + Suc-Ala-Ala-Pro-Phe

Guidance literature:

With (R)-α-<((2S,3S)-2-(9-fluorenylmethyloxycarbonylamino)-3-hydroxybutanoyl)amino>benzenethaneboronic acid; sodium chloride; α-chymotrypsin; N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid; at 25 ℃; Rate constant; other boronic acid inhibitors;

DOI:10.1021/jo9615007

Downstream raw materials:

4-nitro-aniline

Refernces