Chemical Property of (3'R,3aS,4S,4'S,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-(1-amino-2-hydroxyethyl)-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
Chemical Property:
- Appearance/Colour:Amorphous solid or tan powder.
- Melting Point:160-180 °C
- Refractive Index:1.672
- Boiling Point:897.6 °C at 760 mmHg
- PKA:7.1, 8.8(at 25℃)
- Flash Point:496.7 °C
- PSA：272.06000
- Density:1.67 g/cm3
- LogP:-5.47910
- Storage Temp.:2-8°C
- Solubility.:H2O: 50 mg/mL As a stock solution. Stock solutions should b
- Water Solubility.:Miscible with water, methanol, buffer solution and ethanol. Immiscible with less polar solvents.
- XLogP3:-6.6
- Hydrogen Bond Donor Count:11
- Hydrogen Bond Acceptor Count:16
- Rotatable Bond Count:6
- Exact Mass:527.23263824
- Heavy Atom Count:36
- Complexity:756
- Transport DOT Label:Poison
- Purity/Quality:
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98%, *data from raw suppliers
HygromycinBfrom?Streptomyceshygroscopicus?powder,≥90%(HPLC),suitableforcellculture,suitableforinsectcellcultureHazmat ≥90%(HPLC) *data from reagent suppliers
Safty Information:
- Pictogram(s):
T+, 
Xn
- Hazard Codes:T+,Xn,T
- Statements:
26/27/28-41-42/43-40-36-23/24/25
- Safety Statements:
23-26-28-36/37/39-45-22-36-36/37
- MSDS Files:
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SDS file from LookChem
Total 1 MSDS from other Authors
Useful:
- Canonical SMILES:CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N
- Isomeric SMILES:CN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@H]([C@H]([C@H](O4)C(CO)N)O)O)O)O)N
-
Description
Hygromycin B is an aminoglycoside antibiotic that has been found in S. hygroscopicus. It is active against E. coli (MIC = 150 μg/ml) and cytotoxic to both prokaryotic and eukaryotic cells via inhibition of protein synthesis. Hygromycin B has commonly been used in molecular and cell biology applications to select for transformed cells expressing the E. coli hygromycin resistance genes hyg or hph.
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Uses
antibacterial, anthelmintic; LD50 ip (rat) 63mg/Kg Hygromycin B, a selective antibiotic that is effective on most bacteria, fungi and higher eukaryotes, inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome Hygromycin B is an aminoglycoside and a selective antibiotic agent for the hph gene. Hygromycin B inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S rib
osome Hygromycin B is a trisaccharide aminoglycoside with a terminal cyclitol produced by Streptomyces hygroscopicus, reported by researchers at Eli Lily in the early 1950s. Hygromycin B belongs to the destomycin family of aminoglycosides which contain a unique cyclic structure linking the terminal saccharide. Hygromycin B exhibits broad spectrum antibiotic and anthelmintic activity, and is used as a feed additive for swine and poultry. Hygromycin B inhibits polypeptide synthesis by binding peptidyl-tRNA and preventing translocation by Elongation Factor 2.
