5-Cyano-2,3-di-p-tolyltetrazolium chloride

Base Information

• Chemical Name: 5-Cyano-2,3-di-p-tolyltetrazolium chloride
• CAS No.: 90217-02-0
• Deprecated CAS: 102568-47-8
• Molecular Formula: C16H14 N5 . Cl
• Molecular Weight: 311.77
• Hs Code.: 2933998090
• European Community (EC) Number: 600-323-2,622-453-9
• UNII: BUS4P4I6FX
• DSSTox Substance ID: DTXSID90376367
• Wikidata: Q27274896
• Mol file: 90217-02-0.mol

Synonyms:5-CTC cpd;5-cyano-2,3-ditolyl tetrazolium chloride;5-cyano-2,3-ditolyltetrazolium;5-cyano-2,3-ditolyltetrazolium chloride;5-cyano-2,3-tolyl-tetrazolium chloride

Chemical Property of 5-Cyano-2,3-di-p-tolyltetrazolium chloride

Chemical Property:

• Melting Point: 225 ℃
• Boiling Point: °Cat760mmHg
• Flash Point: °C
• PSA: 47.07000
• Density: g/cm³
• LogP: -0.09192
• Storage Temp.: 2-8°C
• Solubility.: Soluble in water
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 2
• Exact Mass: 311.0937732
• Heavy Atom Count: 22
• Complexity: 357

Purity/Quality:

Analysis control,HPLC≥95% ^*data from raw suppliers

5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride ^*data from reagent suppliers

Safty Information:

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Canonical SMILES: CC1=CC=C(C=C1)N2N=C(N=[N+]2C3=CC=C(C=C3)C)C#N.[Cl-]
• Description: 5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable.
• Uses: Redox dye producing a fluorescent formazan (only in solid state) upon reduction. It is used as cellular indicator of respiratory activity in cells, especiall for tracking P450 activity. 5-Cyano-2,3-di-p-tolyltetrazolium Chloride is a monotetrazolium redox dye that is used as a vital stain for actively respiring bacteria. Redox dye producing a fluorescent formazan (only in solid state) upon reduction; used as cellular indicator of respiratory activity in cells.