NTA-FITC

Base Information

• Chemical Name: NTA-FITC
• CAS No.: 1241912-74-2
• Molecular Formula: C31H29N3O11S
• Molecular Weight: 651.64
• Mol file: 1241912-74-2.mol

Synonyms:NTA-FITC;N-[6-(2-BISCARBOXYMETHYLAMINO)-1-CARBOXYPENTYL]-N'-[5-FLUORESCEINYL]THIOUREA

Chemical Property of NTA-FITC

Chemical Property:

• Melting Point: 175-179°C
• Storage Temp.: Amber Vial, -20°C Freezer, Under Inert Atmosphere

Purity/Quality:

NTA-FITC ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

Useful:

• Uses: A fluorescent reagent for the detection of proteins having Histidine-tag (his-tag) A fluorescent reagent for the detection of proteins having Histidine-tag (his-tag).

Technology Process of NTA-FITC

There total 4 articles about NTA-FITC which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 87.0%

C43H53N3O11S → C31H29N3O11S (1241912-74-2)

Guidance literature:

With trifluoroacetic acid; In dichloromethane; at 0 - 20 ℃; for 3.5h; Darkness;

DOI:10.1039/d0cc07095e

Reference yield:

N(ε)-benzoyloxycarbonyl-L-lysine tert-butyl ester hydrochloride (5978-22-3,1426213-63-9) → C31H29N3O11S (1241912-74-2)

Guidance literature:

Multi-step reaction with 4 steps

1: N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 55 °C / Inert atmosphere

2: palladium 10% on activated carbon; hydrogen / methanol

3: N-ethyl-N,N-diisopropylamine; HATU / dichloromethane / 7 h / 20 °C / pH 7 - 8 / Inert atmosphere; Darkness

4: trifluoroacetic acid / dichloromethane / 3.5 h / 0 - 20 °C / Darkness

With palladium 10% on activated carbon; hydrogen; N-ethyl-N,N-diisopropylamine; HATU; trifluoroacetic acid; In methanol; dichloromethane; N,N-dimethyl-formamide;

DOI:10.1039/d0cc07095e

Reference yield:

Nα,Nα-bis[(tert-butyloxycarbonyl)methyl]-Nε-benzyloxycarbonyl-L-lysine tert-butyl ester (205379-07-3) → C31H29N3O11S (1241912-74-2)

Guidance literature:

Multi-step reaction with 3 steps

1: palladium 10% on activated carbon; hydrogen / methanol

2: N-ethyl-N,N-diisopropylamine; HATU / dichloromethane / 7 h / 20 °C / pH 7 - 8 / Inert atmosphere; Darkness

3: trifluoroacetic acid / dichloromethane / 3.5 h / 0 - 20 °C / Darkness

With palladium 10% on activated carbon; hydrogen; N-ethyl-N,N-diisopropylamine; HATU; trifluoroacetic acid; In methanol; dichloromethane;

DOI:10.1039/d0cc07095e

upstream raw materials:

N(ε)-benzoyloxycarbonyl-L-lysine tert-butyl ester hydrochloride

N^α,N^α-bis[(tert-butyloxycarbonyl)methyl]-N^ε-benzyloxycarbonyl-L-lysine tert-butyl ester

N^α,N^α-bis[(tert-butyloxycarbonyl)methyl]-L-lysine tert-butyl ester