Neu5Ac alpha(2-3)Gal beta(1-4)GlcNAc-beta-pNP

Base Information

• Chemical Name: Neu5Ac alpha(2-3)Gal beta(1-4)GlcNAc-beta-pNP
• CAS No.: 501427-92-5
• Molecular Formula: C31H45N3O21
• Molecular Weight: 795.7
• Mol file: 501427-92-5.mol

Synonyms:Neu5Ac alpha(2-3)Gal beta(1-4)GlcNAc-beta-pNP

Chemical Property of Neu5Ac alpha(2-3)Gal beta(1-4)GlcNAc-beta-pNP

Chemical Property:

• Vapor Pressure: 0mmHg at 25°C
• Boiling Point: 1230.868°C at 760 mmHg
• Flash Point: 698.204°C
• PSA: 385.75000
• Density: 1.68g/cm³
• LogP: -3.88090

Purity/Quality:

98% ^*data from raw suppliers

Neu5Acα(2-3)Galβ(1-4)GlcNAc-β-pNP >95.0%(HPLC) ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Uses: 4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-3)-b-D-galactopyranosyl-(1-4)-2-acetamido-2-deoxy-b-D-glucopyranoside (CAS# 501427-92-5) is used in method for determining recognition specificity of virus for receptor sugar chain.

Technology Process of Neu5Ac alpha(2-3)Gal beta(1-4)GlcNAc-beta-pNP

There total 2 articles about Neu5Ac alpha(2-3)Gal beta(1-4)GlcNAc-beta-pNP which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 91.0%

4-nitrophenyl β-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-β-D-glucopyranoside (142925-37-9,142925-38-0,74211-28-2,184377-56-8) + cytidine-5'-monophospho-β-D-N-acetylneuraminic acid, disodium salt → α-D-Neu5Ac-(2->3)-β-D-Gal-(1->4)-β-D-GlcNAc-OC6H4NO2-p (501427-92-5)

Guidance literature:

With recombinant rat α-(2->3)-N-sialyltransferase; alkaline phosphatase; In various solvent(s); at 30 ℃; for 48h; pH=6.3;

DOI:10.1016/j.carres.2005.08.019

Reference yield:

4-nitrophenyl N-acetyl-β-D-glucosaminide (3459-18-5,50645-66-4) → α-D-Neu5Ac-(2->3)-β-D-Gal-(1->4)-β-D-GlcNAc-OC6H4NO2-p (501427-92-5)

Guidance literature:

Multi-step reaction with 2 steps

1: 265.1 mg / Bacillus circulans β-galactosidase / aq. phosphate buffer; acetonitrile / 24 h / 30 °C / pH 6.8

2: 91 percent / alkaline phosphatase; recombinant rat α-(2->3)-N-sialyltransferase / various solvent(s) / 48 h / 30 °C / pH 6.3

With Bacillus circulans β-galactosidase; recombinant rat α-(2->3)-N-sialyltransferase; alkaline phosphatase; In phosphate buffer; acetonitrile;

DOI:10.1016/j.carres.2005.08.019

Reference yield:

α-D-Neu5Ac-(2->3)-β-D-Gal-(1->4)-β-D-GlcNAc-OC6H4NO2-p (501427-92-5) + Bis(sodium) guanosine 5'-β-L-fucopyranosyl-diphosphate (138552-48-4,143394-77-8,148296-47-3) → α-D-Neu5Ac-(2->3)-β-D-Gal-(1->4)-β-D-(α-L-Fuc-(1->3)-)-GlcNAc-OC6H4NO2-p

Guidance literature:

With recombinant human α-(1->3)-fucosyltransferase VII; alkaline phosphatase; In various solvent(s); at 30 ℃; for 20h; pH=7.4;

DOI:10.1016/j.carres.2005.08.019

upstream raw materials:

4-nitrophenyl β-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-β-D-glucopyranoside

4-nitrophenyl N-acetyl-β-D-glucosaminide