Hoechst 33258

Base Information

• Chemical Name: Hoechst 33258
• CAS No.: 23491-45-4
• Deprecated CAS: 53025-60-8,91932-18-2,93038-90-5,863126-37-8,863126-37-8,91932-18-2,93038-90-5
• Molecular Formula: C25H27Cl3N6O
• Molecular Weight: 533.888
• Hs Code.: 32129000
• European Community (EC) Number: 245-690-6
• UNII: MHT095273M
• DSSTox Substance ID: DTXSID5066900
• Wikidata: Q27284034
• ChEMBL ID: CHEMBL34922
• Mol file: 23491-45-4.mol

Synonyms:4-(5-(4-Methyl-1-piperazinyl)(2,5'-bi-1H-benzimidazol)-2'-yl)phenol, trihydrochloride;Bisbenzimidazole;Bisbenzimidazole Trihydrochloride;Bisbenzimide;Hoe 33258;Hoe-33258;Hoe33258;Hoechst 33258;NSC 322921;NSC-322921;NSC322921;Pibenzimol

Chemical Property of Hoechst 33258

Chemical Property:

• Appearance/Colour: yellow to yellow-green powder
• Melting Point: 314 °C
• Refractive Index: 1.6300 (estimate)
• Boiling Point: 643.1oC at 760mmHg
• Flash Point: 342.7oC
• PSA: 84.07000
• Density: 1.5099 (rough estimate)
• LogP: 6.63950
• Storage Temp.: 2-8°C
• Solubility.: H2O: 10 mg/mL
• Water Solubility.: SOLUBLE
• Hydrogen Bond Donor Count: 6
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 3
• Exact Mass: 532.131193
• Heavy Atom Count: 35
• Complexity: 634

Purity/Quality:

98%,99%, ^*data from raw suppliers

Hoechst33258 ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xn
• Hazard Codes: Xn
• Statements: 22-36/38-36/37/38-20/21/22
• Safety Statements: 26-36-24/25-23

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Chemical Classes: Dyes -> Other Dyes
• Canonical SMILES: CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)N=C(N5)C6=CC=C(C=C6)O.Cl.Cl.Cl
• Uses: Hoechst 33258 is useful for staining DNA, chromosomes and nuclei. The nucleic acid binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence Hoechst 33258 is a Hoechst stain used to stain DNA. It is part of a family of fluorescent dyes. Dyes and metabolites. bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.Excitation max. =?346 nmEmission max. =?460 nm