Chemical Property of REVERSE TRANSCRIPTASE, M-MLV, RNASEH+
Chemical Property:
- Purity/Quality:
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98%,99%, *data from raw suppliers
Reverse Transcriptase *data from reagent suppliers
Safty Information:
- Pictogram(s):
- Hazard Codes:
- MSDS Files:
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Useful:
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Description
Enzyme possessed by retroviruses that allows
transcription of DNA from an RNA template. Such an enzyme
has never been isolated from other sources, but speculation
about its possible occurrence continues. Such an enzyme is
widely used experimentally in genetic engineering to allow the
formation of complementary DNA (cDNA) from purified RNA. The discovery of this enzyme faulted the widely held
central dogma, which stated that the information flow in nature
was always from DNA to RNA.
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Uses
Synthesizes a complementary DNA strand using single-stranded RNA or DNA in the presence of a primer M-MLV Reverse Transcriptase has been used:for the preparation of cDNA libraries or for first strand cDNA synthesisfor use in a 2-step RT-PCR assayfor the synthesis of cDNA that is further used in cloning Reverse Transcriptase AMV is suitable for:First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)Dideoxy DNA sequencingPrimer extensionRNA sequencing3′-end labeling of DNA fragmentsGeneration of single-stranded probes for genomic footprint experiments
