THROMBIN

Base Information

• Chemical Name: THROMBIN
• CAS No.: 581-64-6
• Molecular Formula: C12H10 N3 S . Cl
• Molecular Weight: 263.75
• Mol file: 581-64-6.mol

Synonyms:3,7-Diaminophenazathioniumchloride (7CI); Phenothiazin-5-ium, 3,7-diamino-, chloride (8CI,9CI); C.I. 52000;Cyanine; Katalysin; Lauth's violet; Thionin; Thionine

Chemical Property of THROMBIN

Chemical Property:

• Appearance/Colour: dark green to black powder or crystals
• Vapor Pressure: 0.011mmHg at 25°C
• Boiling Point: 271.606°C at 760 mmHg
• Flash Point: 85.966°C
• PSA: 91.00000
• Density: 0.983g/cm³
• LogP: 3.94570
• Storage Temp.: −20°C
• Solubility.: H2O: 1 mL/vial clear to slightly hazy, colorless solution

Purity/Quality:

ThionineChloride ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xn
• Hazard Codes: Xn,B
• Statements: 36/37/38-42
• Safety Statements: 22-24-26-36/37

MSDS Files:

SDS file from LookChem

Useful:

• Description: Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to fibrin and potentiates the procoagulation process by activating factors V, VIII, XI, and XIII. Conversely, thrombin aids the anticoagulant process by activating protein C. Activated protein C inhibits activated factors V and VIII ultimately down-regulating further thrombin production. Thrombin is often used as a potent platelet activator in vitro. Platelet responses to thrombin are mediated through protease-activated receptors (PARs). Thrombin-mediated signaling regulates a diverse range of metabolic processes including activation of phospholipases in platelets, endothelial cells, and cardiac myocytes.
• Uses: Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma. Thionine Chloride is used in the preparation of carbon paste electrode with covalently immobilized thionine for electrochemical sensing of hydrogen peroxide

Technology Process of THROMBIN

There total 6 articles about THROMBIN which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

methylene blue (152071-32-4,105504-42-5,121067-62-7,12262-49-6,167498-52-4,6476-03-5,97130-83-1) → 3,7-diaminophenothiazin-5-ium chloride (581-64-6,93930-11-1) + leucomethylene blue hydrochloride (93798-32-4)

Guidance literature:

With sodium sulfide; oxygen; In water; at 25 ℃; for 0.5h; pH=> 7; Irradiation;

DOI:10.1016/j.saa.2011.05.109

Reference yield:

methylene blue (152071-32-4,105504-42-5,121067-62-7,12262-49-6,167498-52-4,6476-03-5,97130-83-1) → 3,7-diaminophenothiazin-5-ium chloride (581-64-6,93930-11-1)

Guidance literature:

With sodium sulfide; oxygen; In water; at 25 ℃; for 0.5h; pH=6; Irradiation;

DOI:10.1016/j.saa.2011.05.109

Reference yield:

3,7-bis(dimethylamino)phenothiazin-5-ium chloride trihydrate → 4-amino-toluene-2-sulfonic acid (118-88-7) + C12H9NO2S + thionine + 3,7-diaminophenothiazin-5-ium chloride (581-64-6,93930-11-1)

Guidance literature:

With Ba_0.4Sr_0.6Fe₁₂O₁₉; dihydrogen peroxide; In water; for 1h; UV-irradiation;

DOI:10.1016/j.jallcom.2019.153410

upstream raw materials:

1,4-phenylenediamine

leucothionine

methylene blue

Downstream raw materials:

p-benzosemiquinone radical anion