3-[[1-[4-(Diaminomethylideneamino)butylamino]-4-methyl-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylic acid

Base Information

• Chemical Name: 3-[[1-[4-(Diaminomethylideneamino)butylamino]-4-methyl-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylic acid
• CAS No.: 66701-25-5
• Molecular Formula: C15H27N5O5
• Molecular Weight: 357.41
• Hs Code.: 29252900
• European Community (EC) Number: 613-978-4
• Wikipedia: E-64
• Mol file: 66701-25-5.mol

Synonyms:E 64;E-64;L-trans-epoxysuccinyl-leucylamido(4-guanidino)butane;N-(N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucyl)agmatine

Chemical Property of 3-[[1-[4-(Diaminomethylideneamino)butylamino]-4-methyl-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylic acid

Chemical Property:

• Appearance/Colour: white solid
• Melting Point: 182 °C
• Refractive Index: 1.617
• PKA: 3.16±0.40(Predicted)
• PSA: 169.93000
• Density: 1.446 g/cm³
• LogP: 0.72150
• Storage Temp.: 2-8°C
• Sensitive.: Light Sensitive
• Solubility.: H2O: 20 mg/mL, clear, colorless
• Water Solubility.: Soluble in DMSO (25mg/ml), or water (20mg/ml)
• XLogP3: -0.9
• Hydrogen Bond Donor Count: 5
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 11
• Exact Mass: 357.20121898
• Heavy Atom Count: 25
• Complexity: 518

Purity/Quality:

98%,99%, ^*data from raw suppliers

E 64 ^*data from reagent suppliers

Safty Information:

• Safety Statements: 22-24/25

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Canonical SMILES: CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
• Description: E-64 (66701-25-5) is a cell-permeable, epoxysuccinyl peptide irreversible inhibitor of calpain and other cysteine proteases.1,2?Inhibits calpain-dependent apoptosis pathway in T cells3?but promotes heat-induced apoptosis in FM3A cells. Typical working concentration is 0.5-10 μg/ml.4
• Uses: It is used as a broad spectrum cysteine proteinase and calpain activation inhibitor. Additionally, Prevotella intermediate studies report that E-64 blocks the breakdown of methaemoglobin and the oxidation of oxyhaemoglobin by InpA. E-64 has also been used to study excystation in Giardia lamblia. E-64 is an inhibitor of Calpain, cathepsin K and cathepsin S. E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained

Technology Process of 3-[[1-[4-(Diaminomethylideneamino)butylamino]-4-methyl-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylic acid

There total 1 articles about 3-[[1-[4-(Diaminomethylideneamino)butylamino]-4-methyl-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylic acid which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

→ N-(trans-epoxysuccinyl)-L-leucine 4-guanidinobutylamide (66701-25-5)

Guidance literature:

entspr. Ethylester (IX), Hydrolyse (KOH);