Chemical Property of 5-Carboxytetramethylrhodamine succinimidyl ester
Chemical Property:
- PSA：123.43000
- LogP:3.18830
- Storage Temp.:?20°C
- Solubility.:DMF: soluble
- XLogP3:2.6
- Hydrogen Bond Donor Count:0
- Hydrogen Bond Acceptor Count:8
- Rotatable Bond Count:5
- Exact Mass:527.16925015
- Heavy Atom Count:39
- Complexity:1140
- Purity/Quality:
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99%, *data from raw suppliers
5-CarboxytetramethylrhodamineSuccinimidylEster *data from reagent suppliers
Safty Information:
- Pictogram(s):
- Hazard Codes:
- MSDS Files:
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SDS file from LookChem
Useful:
- Canonical SMILES:CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=C(C=C(C=C4)C(=O)ON5C(=O)CCC5=O)C(=O)[O-]
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Description
5-Carboxytetramethylrhodamine succinimidyl ester (5-TAMRA-SE) is an amine-reactive fluorescent probe. It has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays. It quenches lucifer yellow CH fluorescence by greater than 90% in uncleaved proteinase substrates, which allows for the detection of enzyme-cleaved substrates by an increase in fluorescence intensity. It has also been used in the synthesis of fluorescent derivatives of a variety of compounds, including the antibiotic ampicillin , nucleotide diphosphate uridine-5’-diphosphate (UDP; ), and the progesterone receptor antagonist RU486 (mifepristone; ). 5-TAMRA-SE displays excitation maxima ranging from 540 to 560 nm and an emission maximum of 580 nm.
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Uses
Amine-reactive form of 5-carboxytetramethylrhodamine single isomer. Amine-Reactive Fluorescent Probe for labeling of DNA and RNA
