tris-NTA

Base Information

• Chemical Name: tris-NTA
• CAS No.: 862778-60-7
• Molecular Formula: C₄₃H₆₈N₈O₂₂
• Molecular Weight: 1049.05

Synonyms:(S)-5-{8-(6-Amino-hexanoyl)-4,11-bis-[(S)-4-(bis-carboxymethyl-amino)-4-carboxy-butyryl]-1,4,8,11-tetraaza-cyclotetradec-1-yl}-2-(bis-carboxymethyl-amino)-5-oxo-pentanoic acid

Chemical Property of tris-NTA

Chemical Property:

Purity/Quality:

99% ,98%,Electron Grade , ^*data from raw suppliers

Safty Information:

• Pictogram(s):  
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MSDS Files:

Useful:

• Uses: tris-NTA, can be used as a chelating agent for fluorescence labeling of histidine-tagged proteins to lipid membranes, which is a powerful mean for studying and monitoring protein interaction in cells.

Technology Process of tris-NTA

There total 1 articles about tris-NTA which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

5-{8,11-bis[4-bis(tert-butoxycarbonymethyl)amino-4-tert-butoxycarbonylbutyryl]-4-(7-tert-butoxycarbonylaminoheptanoyl)-1,4,8,11-tetraazacyclotetradec-1-yl}-2-bis(tert-butoxycarbonylmethyl)amino-5-oxopentanoic acid tert-butyl ester → (S)-5-{8-(6-Amino-hexanoyl)-4,11-bis-[(S)-4-(bis-carboxymethyl-amino)-4-carboxy-butyryl]-1,4,8,11-tetraaza-cyclotetradec-1-yl}-2-(bis-carboxymethyl-amino)-5-oxo-pentanoic acid (862778-60-7)

Guidance literature:

With ethandithiol; chlorotriisopropylsilane; phenol; trifluoroacetic acid; at 20 ℃; for 4h;

DOI:10.1021/ja050690c

Reference yield:

(S)-5-{8-(6-Amino-hexanoyl)-4,11-bis-[(S)-4-(bis-carboxymethyl-amino)-4-carboxy-butyryl]-1,4,8,11-tetraaza-cyclotetradec-1-yl}-2-(bis-carboxymethyl-amino)-5-oxo-pentanoic acid (862778-60-7) + Oregon Green 488 carboxylic acid, succinimidyl ester, 6-isomer → 2-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)-N-(6-oxo-6-{4,8,11-tris-[4-(bis-carboxymethyl-amino)-4-carboxy-butyryl]-1,4,8,11tetraaza-cyclotetradec-1-yl}-hexyl)-terephthalamic acid

Guidance literature:

With N-ethyl-N,N-diisopropylamine; In N,N-dimethyl-formamide; at 20 ℃; for 12h;

DOI:10.1021/ja0563105

Reference yield:

(S)-5-{8-(6-Amino-hexanoyl)-4,11-bis-[(S)-4-(bis-carboxymethyl-amino)-4-carboxy-butyryl]-1,4,8,11-tetraaza-cyclotetradec-1-yl}-2-(bis-carboxymethyl-amino)-5-oxo-pentanoic acid (862778-60-7) + C35H33N3O7 → C74H96N10O26

Guidance literature:

With N-ethyl-N,N-diisopropylamine; In N,N-dimethyl-formamide; at 20 ℃; for 12h;

DOI:10.1021/ja0563105

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