Flash-edt2

Base Information

• Chemical Name: Flash-edt2
• CAS No.: 212118-77-9
• Deprecated CAS: 960314-82-3
• Molecular Formula: C₂₄H₁₈As₂O₅S₄
• Molecular Weight: 664.511
• DSSTox Substance ID: DTXSID90431400
• Nikkaji Number: J1.745.767E
• Wikipedia: FlAsH-EDT2
• Wikidata: Q1270302
• Mol file: 212118-77-9.mol

Synonyms:4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein;FLASH-EDT2;Lumio Green

Chemical Property of Flash-edt2

Chemical Property:

• Vapor Pressure: 1.02E-30mmHg at 25°C
• Boiling Point: 849.8°C at 760 mmHg
• Flash Point: 467.8°C
• PSA: 177.19000
• LogP: 4.01620
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 9
• Rotatable Bond Count: 2
• Exact Mass: 663.84690
• Heavy Atom Count: 35
• Complexity: 784

Purity/Quality:

98%Min ^*data from raw suppliers

FLASH ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: C1CS[As](S1)C2=C(C=CC3=C2OC4=C(C35C6=CC=CC=C6C(=O)O5)C=CC(=C4[As]7SCCS7)O)O
• Uses: The Fluorescein Arsenical Hairpin Binder (FlAsH) method is presented for fluorescence labeling of proteins in living cells. Labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder-ethanedithiol (FlAsH-EDT2). The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin.

Technology Process of Flash-edt2

There total 2 articles about Flash-edt2 which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 34.0%

fluorescein-4',5 '-bis(mercuric trifluoroacetate) + ethane-1,2-dithiol (540-63-6) → 4'5'-bis(1,2,3-dithioarsolan-2-yl)-fluorescein (212118-77-9)

Guidance literature:

fluorescein-4',5 '-bis(mercuric trifluoroacetate); arsenic trichloride; With N-ethyl-N,N-diisopropylamine; palladium diacetate; In 1-methyl-pyrrolidin-2-one; at 20 ℃;

ethane-1,2-dithiol; In 1-methyl-pyrrolidin-2-one; acetone; for 0.416667h; pH=7; Aqueous phosphate buffer;

Reference yield: 34.0%

fluorescein-4',5'-bis(mercuric trifluoroacetate) (943517-73-5) + ethane-1,2-dithiol (540-63-6) → 4'5'-bis(1,2,3-dithioarsolan-2-yl)-fluorescein (212118-77-9)

Guidance literature:

fluorescein-4',5'-bis(mercuric trifluoroacetate); With arsenic trichloride; palladium diacetate; N-ethyl-N,N-diisopropylamine; at 20 ℃;

ethane-1,2-dithiol; In phosphate buffer; acetone; for 0.333333h;

DOI:10.1021/ja063212q

upstream raw materials:

fluorescein-4',5'-bis(mercuric trifluoroacetate)

ethane-1,2-dithiol