2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate

Base Information

• Chemical Name: 2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate
• CAS No.: 1166837-63-3
• Molecular Formula: C₃₁H₂₂Cl₆O₉
• Molecular Weight: 751.228
• Mol file: 1166837-63-3.mol

Synonyms:

Chemical Property of 2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate

Chemical Property:

Purity/Quality:

97% ^*data from raw suppliers

6-HEXDipivaloate ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

Useful:

• Uses: 6-HEX is an amino-conjugatable fluorescent probe that is widely used in nucleic acid sequencing and related research. It can also be used to label peptides and oligonucleotides.

Technology Process of 2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate

There total 5 articles about 2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 93.0%

→ 2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate (1166837-63-3)

Guidance literature:

With hydrogenchloride; In water;

DOI:10.1016/j.bmcl.2009.04.049

Reference yield: 88.0%

2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate diisopropylamine salt (1166837-64-4) → 2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate (1166837-63-3)

Guidance literature:

With hydrogenchloride; In dichloromethane; water;

DOI:10.1007/s10895-014-1351-x

Reference yield:

3,6-dichloro-1,2,4-benzenetricarboxylic anhydride (81742-10-1) → 2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate (1166837-63-3)

Guidance literature:

Multi-step reaction with 3 steps

1.1: methanesulfonic acid / 28 h / 50 - 110 °C / Inert atmosphere

2.1: 2 h / Reflux

2.2: -20 °C

3.1: hydrogenchloride / dichloromethane; water

With hydrogenchloride; methanesulfonic acid; In dichloromethane; water;

DOI:10.1007/s10895-014-1351-x

upstream raw materials:

3,6-dichloro-1,2,4-benzenetricarboxylic anhydride

2,4-dichlororesorcinol

2',4',5′,7′-tetrachloro-5(6)-carboxy-4,7-dichlorofluorescein

2',4',5',7'-tetrachloro-6-carboxy-4,7-dichlorofluorescein dipivalate diisopropylamine salt