Technology Process of sporulene B
There total 5 articles about sporulene B which
guide to synthetic route it.
The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:
synthetic route:
- Guidance literature:
-
tetraprenyl-ar-curcumene;
With
tetraprenyl-β-curcumene synthase YtpB; edetate disodium; diothiothreitol;
at 30 ℃;
for 1h;
pH=7.5;
Tris*HCl buffer;
Enzymatic reaction;
Heating;
GC-MS instrument;
DOI:10.1021/ja203779h
- Guidance literature:
-
Multi-step reaction with 2 steps
1.1: tetraprenyl-β-curcumene synthase YtpB; edetate disodium; diothiothreitol / 1 h / 30 °C / pH 7.5 / Tris*HCl buffer; Enzymatic reaction
1.2: Heating; GC-MS instrument
2.1: Heating; GC-MS instrument; Enzymatic reaction
With
tetraprenyl-β-curcumene synthase YtpB; edetate disodium; diothiothreitol;
DOI:10.1021/ja203779h
- Guidance literature:
-
Multi-step reaction with 2 steps
1: cells-free extract from E. coli BL21(DE3) harboring the pColdTF-sqhC; diothiothreitol / 16 h / 30 °C / pH 7.5 / Tris*HCl buffer; Enzymatic reaction
2: Heating; GC-MS instrument; Enzymatic reaction
With
cells-free extract from E. coli BL21(DE3) harboring the pColdTF-sqhC; diothiothreitol;
DOI:10.1021/ja203779h