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23491-52-3

Basic Information
CAS No.: 23491-52-3
Name: Hoechst 33342
Article Data: 3
Molecular Structure:
Molecular Structure of 23491-52-3 (Hoechst 33342)
Formula: C27H28 N6 O
Molecular Weight: 452.559
Synonyms: 2,5'-Bibenzimidazole,2'-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)- (8CI); 2-[2-(4-Ethoxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazinyl)benzimidazole;2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole;Bisbenzimide; HOE 33342; Ho 342; Hoechst 33342; NSC 334072
EINECS: 245-691-1
Density: 1.274±0.06 g/cm3(Predicted)
Melting Point: 268oC
Boiling Point: 725.9±70.0 °C(Predicted)
Appearance: Yellow-green powder.
Hazard Symbols:
Risk Codes: 22-37/38
Safety: Mutation data reported. When heated to decomposition it emits toxic vapors of NOx.
PSA: 100.76000
LogP: 7.13970
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    Product ID:TQFR039 Product Name:Hoechst 33342 CAS NO:23491-52-3 Molecular Formula:C27H28N6O · 3HCl · xH2O Molecular Weight:561.93 Purity:≥98% Grade:Ultra Pure Grade Appearance:Yellow powder Application:Commonly used reagents in cytog

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    BisbenzimideH33342trihydrochloride

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Chemistry

IUPAC Name: 2-(4-Ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole
Molecular Formula: C27H28N6O.3(HCl).3(H2O)
Molecular Weight: 452.55g/mol
Freely Rotating Bonds: 5
Polar Surface Area: 73.07 Å2
Flash Point: 420.6 °C
Enthalpy of Vaporization: 114.34 kJ/mol
Boiling Point: 771.9 °C at 760 mmHg
Vapour Pressure: 3.19E-24 mmHg at 25°C 
The Cas Register Number of Bisbenzimide is 23491-52-3 .The chemical synonyms of Bisbenzimide (CAS NO. 23491-52-3) are 33258Hoechst ; 4-(5-(4-Methyl-1-piperazinyl)(2,5’-bi-1h-benzimidazol)-2’-yl)-phenotrihy ; 4-[5-(4-Methyl-1-piperazinyl)[2,5’-bi-1h-benzimidazol]-2’-yl]-phenotrihydr ; Bisbenzimidazoletrihydrochloride ; Bisbenzimidehydrochloride ; Hoe33058;Hoechstdye33258 ; Nsc-322921 .The molecular structure of Bisbenzimide (CAS NO. 23491-52-3) is.

Uses

 It is an organic compound used as a fluorescent stain for DNA in molecular biology applications. It is an intercalating agent between Adenine-thymine base pairs.

Toxicity Data With Reference

1.    

dni-hmn-hla 300 nmol/L

    CRNGDP    Carcinogenesis. 13 (1992),2389.
2.    

dnd-mus-fbr 5 µmol/L

    CRNGDP    Carcinogenesis. 9 (1988),485.
3.    

mnt-ham-ovr 10 µmol/L

    MUREAV    Mutation Research. 448 (2000),35.
4.    

dnd-ham-lng 1 µmol/L

    JHCYAS    Journal of Histochemistry and Cytochemistry. 30 (1982),111.
5.    

dni-ham-lng 1 µmol/L

    JHCYAS    Journal of Histochemistry and Cytochemistry. 30 (1982),111.

Safety Profile

Mutation data reported. When heated to decomposition it emits toxic vapors of NOx.
Hazard Codes:  HarmfulXn
Risk Statements: 22-36/38-36/37/38-20/21/22 
R22: Harmful if swallowed
R36/38:  Irritating to eyes and skin. .
R36/37/38: Irritating to eyes, respiratory system and skin.  
R20/21/22: Harmful by inhalation, in contact with skin and if swallowed.
Safety Statements: 26-36-24/25-23 
S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S36: Wear suitable protective clothing.
S24/25: Avoid contact with skin and eyes 
S23: Do not breathe vapour. 
WGK Germany: 3
RTECS: SM1140500
F: 3-8-10