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| CAS No.: | 3326-34-9 |
|---|---|
| Name: | 5-Aminofluorescein |
| Molecular Structure: | |
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|
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| Formula: | C20H13NO5 |
| Molecular Weight: | 347.327 |
| Synonyms: | Fluorescein,5-amino- (6CI,7CI,8CI);4-Aminofluorescein;Fluoresceinamine isomer 1;Fluoresceinamine-I; |
| EINECS: | 222-043-6 |
| Density: | 1.65 g/cm3 |
| Melting Point: | 223 °C (dec.)(lit.) |
| Boiling Point: | 695.3 °C at 760 mmHg |
| Flash Point: | 374.3 °C |
| Solubility: | Soluble in water (partly), methanol (10 mg/ml), and acetone. |
| Appearance: | Dark red solid |
| Hazard Symbols: |
 Xn
|
| Risk Codes: | 22-36/37/38 |
| Safety: | 26 |
| PSA: | 102.01000 |
| LogP: | 3.82920 |
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Specification
The 5-Aminofluorescein, with CAS registry number 3326-34-9, belongs to the following product categories: (1)Fluorescent Labels and Indicators; (2)Fluorescent Labels & Indicators. It has the systematic name of 5-amino-3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one. The main use of this chemical is biochemical research. And this chemical should be stored at the temperature of 2-8°C.
Physical properties of 5-Aminofluorescein: (1)ACD/LogP: 1.80; (2)# of Rule of 5 Violations: 0; (3)ACD/LogD (pH 5.5): 1.79; (4)ACD/LogD (pH 7.4): 1.77; (5)ACD/BCF (pH 5.5): 13.5; (6)ACD/BCF (pH 7.4): 12.77; (7)ACD/KOC (pH 5.5): 223.4; (8)ACD/KOC (pH 7.4): 211.31; (9)#H bond acceptors: 6; (10)#H bond donors: 4; (11)#Freely Rotating Bonds: 3; (12)Polar Surface Area: 57.23 Å2; (13)Index of Refraction: 1.819; (14)Molar Refractivity: 91.61 cm3; (15)Molar Volume: 210.5 cm3; (16)Polarizability: 36.31×10-24cm3; (17)Surface Tension: 101.1 dyne/cm; (18)Enthalpy of Vaporization: 105.48 kJ/mol; (19)Vapour Pressure: 5.66E-20 mmHg at 25°C.
Preparation: this chemical can be prepared by 3',6'-dihydroxy-5-nitro-spiro[phthalan-1,9'-xanthen]-3-one. This reaction will need reagents NaSH, Na2S.9H2O and solvent H2O. The reaction time is 24 hour(s). The yield is about 77%.
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Uses of 5-Aminofluorescein: it can be used to produce N-(5-Fluoresceinyl)maleamic acid. This reaction will need reagent AcOH. The reaction time is 4.0 hour(s). The yield is about 92%.
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When you are using this chemical, please be cautious about it as the following:
The 5-Aminofluorescein irritates to eyes, respiratory system and skin. And this chemical is harmful if swallowed. When use it, wear suitable protective clothing, gloves and eye/face protection. If contact with eyes, rinse immediately with plenty of water and seek medical advice.
You can still convert the following datas into molecular structure:
(1)SMILES: O=C2OC4(c1ccc(N)cc12)c5ccc(O)cc5Oc3cc(O)ccc34
(2)InChI: InChI=1/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
(3)InChIKey: GZAJOEGTZDUSKS-UHFFFAOYAS
(4)Std. InChI: InChI=1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
(5)Std. InChIKey: GZAJOEGTZDUSKS-UHFFFAOYSA-N